Effects of piperine on experimental colon carcinogenesis induced by DMH/DSS and its mechanism
10.3760/cma.j.cn431274-20210627-00695
- VernacularTitle:胡椒碱对DMH/DSS诱导实验性结肠癌变的影响及其机制
- Author:
Yidong LI
1
;
Tingting LI
;
Zhufeng ZHAO
;
Guozhong WU
Author Information
1. 上海杉达学院国际医学技术学院基础医学部，上海 201209
- Keywords:
Colorectal neoplasms;
Piperine;
RAS/PI3K/AKT signal axis;
Dimethylhydrazine;
Sodium dextran sulfate
- From:
Journal of Chinese Physician
2022;24(10):1493-1497,1503
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of piperine on experimental colon carcinogenesis induced by 1, 2-dimethylhydrazine (DMH)/sodium dextran sulfate (DSS) and its mechanism.Methods:36 mice were divided into control group, model group and piperine group, 12 mice in each group. The control group was given normal saline by gavage; The model group and piperine group were given 3.6 mg/(kg·d) of normal saline and piperine respectively after establishing the experimental colon cancer model induced by DMH/DSS. Tumor load and volume were observed. Hematoxylin and eosin (HE) staining was used to observe the histological change of colon in mice. RAS/PI3K/AKT related pathway protein expression was detected by Western blot.Results:The body weight gain, protein expression levels of cleaved poly-ADP ribose polymerase (PARP), cleaved caspase-3 in model group were significantly lower than those in the control group (all P<0.05). The protein expression levels of Bcl-2, Bax, pan-Ras, p-MEK, p-ERK, PI3K, p-AKT, NF-κBP65, c-Myc and cyclin D1 in model group were significantly higher than those in the control group (all P<0.05). The body weight gain, protein expression levels of cleaved PARP and cleaved caspase-3 in piperine group were significantly higher than those in model group (all P<0.05). The protein expression levels of Bcl-2, Bax, pan-Ras, p-MEK, p-ERK, PI3K, p-AKT, NF-κBP65, c-Myc and cyclin D1 in piperine group were significantly lower than those in model group (all P<0.05). Conclusions:Piperine can inhibit the occurrence of experimental colon cancer induced by DMH/DSS, which may involve multiple components of RAS/PI3K/AKT signal axis.
