Effects of GS-Rg1 on secretion of exosomes and expression of angiogenesis related miRNAs in mesenchymal stem cells
10.3760/cma.j.cn431274-20220317-00192
- VernacularTitle:人参皂苷调控间充质干细胞分泌外泌体及对血管新生相关miRNAs表达的影响
- Author:
Saiping MAO
1
;
Zilin CHEN
;
Xi ZHANG
;
Meixin TAN
;
Ailin HU
;
Wei TANG
;
Qianpei YANG
;
Wu XIONG
Author Information
1. 湖南师范大学附属长沙市妇幼保健院产科，长沙 410007
- Keywords:
Mesenchymal stromal cells;
Exosomes;
Neovascularization;
Ginsenoside;
MicroRNAs;
In vitro
- From:
Journal of Chinese Physician
2022;24(9):1312-1315,1320
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of ginsenoside Rg1 (GS-Rg1) on the secretion of exosomes (MSC-Exo) and expression of angiogenesis related microRNAs (miRNAs) in mesenchymal stem cells.Methods:Human umbilical cord blood mesenchymal stem cells (hUCBMSCs) were divided into experimental group and control group. The experimental group was treated with GS-RG1 at a final concentration of 40 mg/L, while the control group was treated with phosphate buffered saline (PBS) at the same volume. Both groups were cultured for 24 h. The morphology of MSC-Exo was observed by transmission electron microscopy; the characteristic surface markers were identified by Western blot; the concentration of MSC-Exo was detected by dicootanobutyric acid protein quantification method, and the expression of 8 miRNAs related to angiogenesis in MSC-Exo was detected by reverse transcription polymerase chain reaction (RT-PCR).Results:After 24 h of incubation, MSC-Exo with a circular membrane vesicle structure was visible. MSC-Exo was positive for the expression of the characteristic surface markers CD9, CD63 and TSG101. After 24 h of intervention, the concentration of MSC-Exo protein were (1.080±0.019)μg/μl and (0.881±0.032)μg/μl in the experimental group and control group, respectively, with statistically significant difference ( P<0.01). The expression of miR-126-3p, miR-21, miR-146a-5p and miR-125b-5p in the GS-Rg1 group were significantly higher than that in the control group, while the expression of miR-16-5p was significantly lower than that in the control group (all P<0.05). Conclusions:GS-Rg1 promotes the secretion of MSC-Exo and enhances the expression of angiogenesis-related miRNAs within Exo to promote angiogenesis.
