Observation of the growth and development of subchondral bone in rat condyles exposed to fluoride at the level of bone microarchitecture
10.3760/cma.j.cn231583-20211009-00342
- VernacularTitle:骨微结构水平观察染氟对大鼠髁突软骨下骨生长发育的影响
- Author:
Chun LIU
1
;
Ying JIA
;
Shirong YANG
;
Qi DING
;
Hua YANG
;
Bo CHEN
Author Information
1. 贵州医科大学口腔医学院口腔解剖学教研室，贵阳 550004
- Keywords:
Fluorine;
Micro CT;
Condyle;
Subchondral bone;
Bone microarchitecture
- From:
Chinese Journal of Endemiology
2022;41(8):626-633
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of fluoride on growth and development of bone microstructure of rats condyle subchondral bone (RCSB).Methods:Forty two 3-week-old SD rats (half male and half female) were fed adaptively for 1 week, and 3 females and 3 males were sacrificed and recorded as 0 month. The remaining rats were randomly divided into control group ( n = 18) and fluoride exposed group ( n = 18) according to their body weight (55 - 70 g), half male and half female. The fluoride exposed group was fed with 150 mg/L sodium fluoride (NaF) aqueous solution, and the control group was fed with tap water. The two groups of experimental animals were sacrificed at 3, 5 and 7 month, respectively, 6 rats in each group, half male and half female. The right mandibular condyle was separated, and Micro CT scanning was performed to detect the microstructure parameters of RCSB. Results:In fluoride exposed group (3 month), bone surface/tissue volume (BS/TV), bone surface/bone volume (BS/BV), trabecular thickness (Tb.Th), trabecular number (Tb.N), structure model index (SMI), connectivity, connectivity density (Conn.D) and total porosity of female rats were significantly different from those of male rats ( t = - 5.10, - 5.58, 4.52, - 4.32, - 4.03, - 2.81, - 6.71, - 3.32, P < 0.05). There was no significant difference in each index between female and male rats in fluoride exposed group (5, 7 month, P > 0.05). Conclusion:In chronic fluorine exposure bone environment, the RCSB bone microarchitecture of male and female rats is different with time, showing the tendency of fluoride injury that the bone changes of female rats are slowed and the bone changes of male rats are active.
