Multiple rapid-responsive probes for hypochlorite detection based on dioxetane luminophore derivatives
- Author:
Sun YINGAI
1
;
Gao YUQI
;
Tang CHUNCHAO
;
Dong GAOPAN
;
Zhao PEI
;
Peng DUNQUAN
;
Wang TIANTIAN
;
Du LUPEI
;
Li MINYONG
Author Information
1. Department of Medicinal Chemistry,Key Laboratory of Chemical Biology(MOE),School of Pharmacy,Shandong University,Jinan,250012,China
- Keywords:
Glow-type;
Chemiluminescent probe;
1,2-Dioxetane;
Hypochlorite
- From:
Journal of Pharmaceutical Analysis
2022;12(3):446-452
- CountryChina
- Language:Chinese
-
Abstract:
In recent years,various methods for detecting exogenous and endogenous hypochlorite have been studied,considering its essential role as a biomolecule.However,the existing technologies still pose obstacles such as their invasiveness,high costs,and complicated operation.In the current study,we developed a glow-type chemiluminescent probe,hypochlorite chemiluminescence probe(HCCL)-1,based on the scaffold of Schaap's 1,2-dioxetane luminophores.To better explore the physiological and pathological functions of hypochlorite,we modified the luminophore scaffold of HCCL-1 to develop several probes,including HCCL-2,HCCL-3,and HCCL-4,which amplify the response signal of hypo-chlorite.By comparing the luminescent intensities of the four probes using the IVIS? system,we determined that HCCL-2 with a limit of detection of 0.166 μM has enhanced sensitivity and selectivity for tracking hypochlorite both in vitro and in vivo.
