The diagnostic value of tuberculin skin test, tubercle bacillus-antibody, interferon-γ release assay, tubercle bacillus-DNA and bacterial collection centrifugal smear method in pneumoconiosis combined with tuberculosis
10.3760/cma.j.cn115455-20220412-00284
- VernacularTitle:皮肤结核菌素试验、结核分枝杆菌抗体、γ-干扰素释放试验、结核分枝杆菌核酸和集菌离心涂片法对尘肺合并结核诊断价值探究
- Author:
Zhiwei WU
1
;
Xiuhua ZHANG
;
Zhiwei YAN
;
Na CHEN
;
Chenyong WEN
;
Baoli XIANG
Author Information
1. 张家口市肺科医院呼吸内科，张家口 075441
- Keywords:
Pneumoconiosis;
Tuberculosis;
Tuberculin skin test;
Tubercle bacillus-antibody;
Interferon-γ release assay
- From:
Chinese Journal of Postgraduates of Medicine
2022;45(10):883-888
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate five kinds of tuberculin skin test (TST), tubercle bacillus-antibody(TB-Ab), interferon-γ release assay(IGRA), tubercle bacillus-DNA (TB-DNA) and collection of bacterial centrifugal smears methods, the application value of combined detection in improving the diagnostic efficiency of pneumoconiosis complicated with tuberculosis.Methods:A total of 150 eligible patients with pneumoconiosis from January 2018 to January 2019 in Zhangjiakou Pulmonary Hospital were selected as the research subjects, and all of them underwent TST, TB-Ab, IGRA, TB-DNA and bacterial centrifugal smear detection. Compared the positive rates of five detection methods in pneumoconiosis and its different stages, and compare the proportion of tuberculosis infection and tuberculosis in different stages of pneumoconiosis.Results:Among the 150 patients with pneumoconiosis, 41 cases (27.33%) were with pneumoconiosis complicated with tuberculosis infection, 24 cases (16.00%) with pneumoconiosis complicated with clinically diagnosed pulmonary tuberculosis, 21 cases (14.00%) with pneumoconiosis complicated with confirmed pulmonary tuberculosis, and 45 cases (30.00%) with pneumoconiosis complicated with pulmonary tuberculosis; with the improvement of pneumoconiosis stage, the proportion of pneumoconiosis combined with tuberculosis infection and pulmonary tuberculosis increased significantly ( P<0.05). Compared with TB-Ab, TB-loop-mediated isothermal amplification(LAMP), and interlayered cup collection centrifuge smear method, the overall positive rate of IGRA detection and pneumoconiosis stage Ⅲ were higher ( P<0.05), but there was no significant difference compared with TST detection ( P>0.05). The positive rate of combined detection was higher, but there was no significant difference compared with IGRA detection ( P>0.05). With the increase of pneumoconiosis stage, the positive reaction intensity of TST decreased, and the positive value of TB-Ab and IGRA increased. Conclusions:The combined detection of TST, TB-Ab, IGRA, TB-DNA and bacterial centrifugal smear method can significantly improve the diagnostic efficiency of pneumoconiosis combined with tuberculosis.
