Isolation and identification of human placental microvascular endothelial cells
10.3760/cma.j.cn115455-20200701-00822
- VernacularTitle:胎盘微血管内皮细胞的分离与鉴定
- Author:
Lili LIU
1
;
Yuyan LI
;
Xueling QU
Author Information
1. 大连市妇幼保健院产科，大连 116033
- Keywords:
Microvascular endothelial cells, placenta;
Isolation;
Identification
- From:
Chinese Journal of Postgraduates of Medicine
2022;45(8):749-752
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To isolate and identify the phenotype and biological characteristics of human placental microvascular endothelial cells(HPMEC).Methods:The HPMEC were isolated from term placenta and cultured in ECM medium. The morphology of the cells was observed under microscope. The functions of HPMEC were detected by uptake of Dil-Ac-LDL, tube formation and cell proliferation. Western Blot was used to detect the expression of endothelial nitric oxide synthase (eNOS), PROKR1 and PROKR2.Results:The HPMEC isolated from the placenta could expressed eNOS, PROKR1 and PROKR2, and were capable of the functions of Dil-Ac-LDL uptaking, tube formation, cell proliferation.Conclusions:HPMEC can be isolated from the placenta, proliferate effectively in vitro and maintain the characteristics of endothelial cells.
