The alleviating effects of endothelial progenitor cells-derived exosomes on hyperoxia-induced injury of type Ⅱ alveolar epithelial cells in neonatal rats
10.3760/cma.j.issn.2096-2932.2022.05.014
- VernacularTitle:内皮前体细胞来源外泌体减轻新生大鼠肺泡Ⅱ型上皮细胞高氧损伤的研究
- Author:
Wen ZENG
1
;
Jin MAO
;
Xuhong HU
;
Shuqiang GAO
;
Rong JU
Author Information
1. 电子科技大学医学院附属妇女儿童医院·成都市妇女儿童中心医院新生儿科，成都 611731
- Keywords:
Endothelial progenitor cell;
Exosomes;
Type Ⅱ alveolar epithelial cell;
Hyperoxia;
Apoptosis
- From:Chinese Journal of Neonatology
2022;37(5):452-456
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of endothelial progenitor cells (EPC)-derived exosomes on hyperoxia-induced injury in type Ⅱ alveolar epithelial cell (AECⅡ) in neonatal rats.Methods:EPCs of rats were cultured and exosomes were collected using Total Exosome Isolation kit. Primary cultured AECⅡof neonatal rats were randomly assigned into three groups: the control group, the hyperoxia group and the exosome group. The control group was cultured in room air with 5%CO 2, the hyperoxia group was cultured in 95%O 2 with 5%CO 2 and the exosome group was cultured with 0.1 mg/ml EPC-derived exosomes in 95%O 2 with 5%CO 2. Cell viability was detected using cell counting kit-8 (CCK-8) and apoptosis was detected using flow cytometry on d2, d4, and d6. Results:EPC-derived exosomes isolated from EPC culture supernatant were confirmed morphologically using transmission electron microscopy. After co-incubation of Dil-labeled EPC-derived exosomes with AEC Ⅱ for 24 h, Dil fluorescence was detected in the cytoplasm of AEC Ⅱ, indicating exosomes were uptaken by AEC Ⅱ. Compared with the control group, hyperoxia decreased cell viability and increased apoptosis of AEC Ⅱ and the injury was aggravated with the prolongation of hyperoxia duration ( P<0.001). Cell injury in the exosome group was milder than the hyperoxia group ( P<0.001). Compared with the control group, cell viability on d4 and d6 of hyperoxia was lower ( P=0.029 and 0.005 respectively) and cell apoptosis at d6 of hyperoxia was higher in the exosome group ( P=0.007). Conclusions:EPC-derived exosomes may partially attenuate hyperoxia-induced cell injury in neonatal rat AEC Ⅱ.
