Rapid detection of pneumocystis carinii infection in immunocompromised patients by loop mediated isothermal amplification technique
10.3760/cma.j.issn.1673-4912.2022.09.009
- VernacularTitle:环介导等温扩增技术在免疫功能低下患儿中快速检测耶氏肺孢子菌感染的研究
- Author:
Jing WANG
1
;
Yajuan ZHOU
;
Lijuan LUO
;
Wenjuan CHEN
;
Qing CAO
;
Wei WANG
Author Information
1. 上海交通大学医学院附属上海儿童医学中心感染科 200127
- Keywords:
Loop mediated isothermal amplification;
Immunocompromised patients;
Pneumocystis carinii;
Pathogen detection
- From:
Chinese Pediatric Emergency Medicine
2022;29(9):707-711
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the value of detecting pneumocystis carini(PC)rapidly in immunocompromised patients by loop mediated isothermal amplification(LAMP).Methods:Respiratory tract specimens of immunocompromised children suspected of pneumocystis carinii pneumonia(PCP) at Shanghai Children′s Medical Center, School of Medicine, Shanghai Jiao Tong University were collected from May 2020 to May 2021.PCR and LAMP methods were used to detect PC.Firstly, LAMP primers of PC were synthetized according to the conserved region of PC gene, and the LAMP reaction system and reaction conditions were optimized to evaluate the sensitivity and specificity.Then, the results of pathogens were compared with those of PCR detection.Results:The established LAMP detection technology for PC had high specificity and super sensitivity.The detection results could be obtained within 1 hour.In 12 clinical samples, 10 cases were positive and 2 cases were negative, the coincidence rate of LAMP and PCR technique was 100%.Conclusion:LAMP can detect PC more rapidly and sensitively than PCR, and it can provide a good support for clinical rapid diagnosis of PCP.
