Noxious electrical stimulation of the pelvic floor and vagina induces transient voiding dysfunction in a rabbit survival model of pelvic floor dystonia.
10.4111/kju.2015.56.12.837
- Author:
Amy D DOBBERFUHL
1
;
Sara SPETTEL
;
Catherine SCHULER
;
Robert M LEVIN
;
Andrew H DUBIN
;
Elise J B DE
Author Information
1. Department of Urology, Stanford University School of Medicine, Stanford, CA, USA. adobber@stanford.edu
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Animal models;
Dystonia;
Pelvic floor;
Urination;
Vagina
- MeSH:
Animals;
Disease Models, Animal;
Dystonia/*etiology;
Electric Stimulation/adverse effects/methods;
Electromyography/methods;
Female;
Muscle Contraction/physiology;
Pelvic Floor/*physiopathology;
Pelvic Floor Disorders/*complications/physiopathology;
Rabbits;
Urinary Bladder/physiopathology;
Urinary Retention/*etiology;
Urination/physiology;
Urine;
Vagina/*physiopathology
- From:Korean Journal of Urology
2015;56(12):837-844
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Existing data supports a relationship between pelvic floor dysfunction and lower urinary tract symptoms. We developed a survival model of pelvic floor dysfunction in the rabbit and evaluated cystometric (CMG), electromyographic (EMG) and ambulatory voiding behavior. MATERIALS AND METHODS: Twelve female adult virgin rabbits were housed in metabolic cages to record voiding and defecation. Anesthetized CMG/EMG was performed before and after treatment animals (n=9) received bilateral tetanizing needle stimulation to the pubococcygeous (PC) muscle and controls (n=3) sham needle placement. After 7 days all animals were subjected to tetanizing transvaginal stimulation and CMG/EMG. After 5 days a final CMG/EMG was performed. RESULTS: Of rabbits that underwent needle stimulation 7 of 9 (78%) demonstrated dysfunctional CMG micturition contractions versus 6 of 12 (50%) after transvaginal stimulation. Needle stimulation of the PC musculature resulted in significant changes in: basal CMG pressure, precontraction pressure change, contraction pressure, interval between contractions and postvoid residual; with time to 3rd contraction increased from 38 to 53 minutes (p=0.008 vs. prestimulation). Vaginal noxious stimulation resulted in significant changes in: basal CMG pressure and interval between contractions; with time to 3rd contraction increased from 37 to 46 minutes (p=0.008 vs. prestimulation). Changes in cage parameters were primarily seen after direct needle stimulation. CONCLUSIONS: In a majority of animals, tetanizing electrical stimulation of the rabbit pelvic floor resulted in voiding changes suggestive of pelvic floor dysfunction as characterized by a larger bladder capacity, longer interval between contractions and prolonged contraction duration.
