The expression and clinical significance of promyelocytic leukemia zinc finger in human peripheral blood with asthma
10.3760/cma.j.issn.1671-0282.2022.10.016
- VernacularTitle:早幼粒细胞白血病锌指在哮喘患者外周血中的表达及意义
- Author:
Xiaoting REN
1
;
Tianyun SHI
;
Na LI
;
Yanchao HE
;
Ling QIAN
;
Jingjing FENG
;
Zhoufang MEI
;
Zhijun JIE
Author Information
1. 复旦大学附属上海市第五人民医院呼吸与危重症医学科 复旦大学社区健康研究中心（筹）,上海 200240
- Keywords:
Transcription factor PLZF;
Asthma;
iNKT;
PBMC;
Peripheral blood;
ELISA;
qPCR;
Flow cytometry
- From:
Chinese Journal of Emergency Medicine
2022;31(10):1389-1395
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of promyelocytic leukemia zinc finger (PLZF) in human peripheral blood with asthma and its clinical significance.Methods:Forty patients with stable asthma from May 2021 to October 2021 in the Department of Respiratory Medicine of the Shanghai Fifth People's Hospital were enrolled, and forty healthy controls were recruited in the study. The levels of cytokines in serum were measured by enzyme-linked immunosorbent assay (ELISA). Quantitative real-time PCR (qPCR) was used to detect the expression of PLZF mRNA in plasma. The level and distribution of PLZF+ cells in PBMCs were detected by flow cytometry after isolating peripheral blood mononuclear cells (PBMCs). Independent sample t test, Mann-Whitney U test, χ 2 test, ROC curve and Logistic regression were used to analyze the results with SPSS 26.0 and Graphpad Prism 7.0. A P<0.05 was considered statistically significant. Results:The levels of cytokines IFN-γ, IL-2, IL-4, TNF-α and IL-17 in human peripheral blood from the asthma group were obviously higher than those in the control group ( P<0.05), whereas there was no significant difference in the level of cytokine IL-10 between the two groups. The level of PLZF mRNA in PBMCs from the asthma group was significantly up-regulated compared to that in the control group [(3.40%±2.52%) vs. (1.23%±0.78%), P<0.05]. CD8+PLZF+ and Vβ11+PLZF+T cells in the asthma group were significantly outnumbered than those in the control group ( P<0.05). Logistic regression and ROC curve analysis showed that PLZF expression in PBMC was a risk factor for the development of asthma ( OR =3.67, AUC=0.87, P<0.05). Conclusions:The high expression of PLZF in peripheral blood may play an important role in the development of asthma, which needs to be further confirmed by large sample studies.
