Effect of Xijiao Dihuang decoction on microRNA expression in liver tissue of septic mice
10.3760/cma.j.issn.1671-0282.2022.10.008
- VernacularTitle:犀角地黄汤对脓毒症小鼠肝组织microRNA表达的影响
- Author:
Mingrui LIN
1
;
Cuifang ZHANG
;
Biqing ZHENG
;
Huaiyu CHEN
;
Xiaoyan GUO
;
Wei LI
Author Information
1. 福建中医药大学附属人民医院重症医学科，福州 350004
- Keywords:
Xijiao Dihuang Decoction;
Sepsis;
Liver;
microRNA
- From:
Chinese Journal of Emergency Medicine
2022;31(10):1341-1346
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of Xijiao Dihuang Ddecoction (XJDHT) against sepsis-induced liver injury based on transcriptomics.Methods:Sixty C57BL/6 mice were randomly (random number) divided into the sepsis group, sepsis treatment with XJDHT and control group, with 20 mice in each group. The sepsis mouse model was established by intraperitoneal (i.p.) injection of lipopolysaccharide (LPS). The control group was intraperitoneally injected with the same amount of normal saline. The sepsis treatment with XJDHT group was injected with XJDHT (crude drug 187.5 mg) twice a day 2 days before modeling. After modeling, gastric feeding was continued twice a day, while the control group and sepsis group were gavaged with the same amount of normal saline. At 72 h after LPS intervention, 9 mice in each group were randomly selected. After anesthesia, part of the liver were taken for small RNA and RNA sequencing and analysis, and part of the liver were taken for pathological examination.Results:XJDHT could improve the histopathological changes of liver in septic mice, and alleviate some abnormally expressed microRNAs (mmu-mir-292a-5p, mmu-mir-871-3p, mmu-mir-653-5p, mmu-mir-293-5p, mmu-mir-155-3p, mmu-mir-346-5p, mmu-mir-187-5p, mmu-mir-3090-3p) and their target genes.Conclusions:XJDHT can reduce the liver histopathological changes in septic mice, and its mechanism may be related to XJDHT regulating the expression of important key genes of liver of sepsis like mmu-mir-187-5p and its target genes such as ADAM8, irak3 and PFKFB3
