Protective effect of hydroxysafflor yellow A on lung injury associated with severe acute pancreatitis in mice
10.3760/cma.j.issn.1671-0282.2022.06.016
- VernacularTitle:羟基红花黄色素A对小鼠重症急性胰腺炎相关肺损伤的保护作用研究
- Author:
Jin ZHAO
1
;
Lichao SUN
;
Wenjing WU
;
Jianping YANG
;
Yiqiang XIE
;
Liuwei ZHANG
;
Meijia SHEN
Author Information
1. 卫健委中日友好医院普外科乳甲外科，北京 100029
- Keywords:
Hydroxysafflor yellow A;
Severe acute pancreatitis;
Inflammatory response;
Oxidative stress;
Lung injury;
NF-κB;
Mechanism;
Drug target
- From:
Chinese Journal of Emergency Medicine
2022;31(6):789-793
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect and mechanism of hydroxysafflor yellow A (HSYA) on severe acute pancreatitis (SAP) related lung injury.Methods:Fifty mice were randomly (random number) divided into five groups: the sham-operated group, SAP group and different doses (20, 40 and 80 mg/kg) of HSYA pretreatment group. Mice were pretreated with HSYA 24 h before SAP induction, pancreatic and lung tissues were isolated for histopathological examination at 72 h after modeling, and bronchoalveolar lavage fluid (BALF) was collected for biochemical analysis. Results:Compared with the sham-operated group, serum amylase activity, lung injury pathological score and BALF protein concentration in the SAP group were significantly increased [(2120.44 ± 354.50) U/L vs. (226.72 ± 20.84) U/L; (6.91 ± 0.28) vs. (0.53±0.18); (2563.25±348.22) μg/mL vs. (345.62±56.35) μg/mL, all P<0.05]. Inflammatory factors tumor necrosis factor (TNF)-α and interleukin (IL)-6 levels and myeloperoxidase (MPO) activity were increased [(120.5±14.25) pg/mL vs. (31.5±4.82) pg/mL; (214.72±10.62) pg/mL vs. (39.26±5.66) pg/mL; (4.52±0.34) U/mg vs. (1.03±0.17) U/mg]. Compared with the SAP group, HSYA pretreatment significantly attenuated SAP-related pancreatic and lung tissue damage and the activities of the inflammatory factors TNF-α, IL-6 and MPO in BALF. In addition, HSYA promoted the expression of the antioxidant protein heme oxygenase-1 and blocked the activation of the NF-κB signaling pathway. Conclusions:HSYA exerts anti-inflammatory and antioxidant activities to inhibit SAP-related lung injury, which indicated that HSYA may be a potential therapeutic drug for SAP-induced lung injury.
