Correlation between protein C -1641A/-1654C haplotype and coagulation disorder in sepsis
10.3760/cma.j.issn.1671-0282.2022.06.013
- VernacularTitle:蛋白C -1641A/-1654C单倍型与脓毒症凝血功能的相关性研究
- Author:
Yan ZHANG
1
;
Hui YE
;
Qixing CHEN
;
Qiang SHU
Author Information
1. 浙江大学医学院附属儿童医院 国家儿童健康与疾病临床医学研究中心，杭州 310052
- Keywords:
Sepsis;
Activated protein C;
Direct sequencing;
Haplotype;
Correlation analysis;
Coagulation disorder;
Platelet count;
Partial activated thrombin time
- From:
Chinese Journal of Emergency Medicine
2022;31(6):773-776
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the correlation between protein C -1641A/-1654C haplotype and coagulation disorder in Chinese Han septic patients.Methods:The genotypes of protein C gene -1641A>G (rs1799809) and -1654C>T (RS1799808) in septic patients were detected by direct sequencing, and their haplotypes were analyzed and divided into two groups according to the haplotype, -1641A/-1654C (AC) carriers and non-AC haplotype carriers. At the same time, unpaired t test or Mann-Whitney U test was used to compare the differences in coagulation/fibrinolytic parameters, including partial activated thrombin time, prothrombin time, internationally standardized ratio of prothrombin time, thrombin time, fibrinogen and D-dimer levels, as well as APC levels between the two groups. Results:A total of 174 septic patients were included in this study, including 60 AC haplotype carriers and 114 non-AC haplotype carriers. Compared with non-AC haplotype carriers, AC haplotype carriers had significantly lower platelet counts, significantly longer partial activated thrombin time, and significantly decreased activated protein C levels. Other coagulation/fibrinolytic parameters including prothrombin time, internationally standardized ratio of prothrombin time, thrombin time, fibrinogen and D-dimer were not significantly different between the two groups.Conclusions:In this study, the protein C-1641A/-1654C haplotype was found to lead to decreased circulating activated protein C levels decreased platelet counts, and prolonged partial activated thrombin time in septic patients. These results suggest that the protein C-1641A/-1654C haplotype may directly affect the APC level and consequently influence the coagulation disorder of sepsis.
