Establishment of fingerprints of Coptidis Rhizoma-Magnoliae Officmalis Cortex for formula granule and determination of index components
10.3760/cma.j.cn115398-20220808-00085
- VernacularTitle:黄连-厚朴药对配方颗粒指纹图谱的建立及指标成分含量测定研究
- Author:
Dongyun ZHAO
1
;
Xudong CHENG
;
Yifei WU
;
Yuyu HUANG
Author Information
1. 安徽中医药大学药学院，合肥 230012
- Keywords:
Couplet medicines;
Coptidis Rhizoma;
Magnoliae Officmalis Cortex;
Formula granule;
Fingerprint;
Assay (TCD)
- From:
International Journal of Traditional Chinese Medicine
2022;44(12):1402-1407
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the HPLC fingerprints of Coptidis Rhizoma- Magnoliae Officmalis Cortex formula granules and the differences of active ingredients in different proportions; To explore the content changes of key components in different proportions of Coptidis Rhizoma- Magnoliae Officmalis Cortex. Methods:HPLC was used to determine the contents of several alkaloids and total phenol of Magnolia officinalis in Coptidis Rhizoma- Magnoliae Officmalis Cortex formula granules and their fingerprints, and the similarity evaluation, cluster analysis and principal component analysis were performed. Results:The similarity of fingerprint of 10 batches of Coptidis Rhizoma- Magnoliae Officmalis Cortex was > 0.950. 17 common peaks were identified, and 6 components were identified. Compared with single medicine, the contents of alkaloids and total phenols in the Coptidis Rhizoma- Magnoliae Officmalis Cortex formula granules were significantly reduced. The contents of multiple alkaloids and total phenols in the Coptidis Rhizoma- Magnoliae Officmalis Cortex formula granules in different proportions were different, and the contents of alkaloids and total phenols were the highest when the proportion of Coptidis Rhizoma- Magnoliae Officmalis Cortex was 2∶1. Conclusion:The contents of main components of Coptidis Rhizoma- Magnoliae Officmalis Cortex formula granules with different proportions are different, which can provide a certain basis for studying the compatibility mechanism of TCM couplet medicines.
