Effects of abdominal massage on the exercise and balance of rats with neonatal hypoxic-ischemic brain injury via 5-HTR1A/cAMP/PKA signaling pathway
10.3760/cma.j.cn115398-20220524-00318
- VernacularTitle:腹部推拿通过5-HTR1A/cAMP/PKA信号通路对新生缺氧缺血性脑损伤大鼠运动平衡功能的影响
- Author:
Wei WANG
1
;
Chutao CHEN
;
Kun AI
;
Xiqin YI
;
Yumei HUANG
;
Qianru CAO
;
Dejun WANG
Author Information
1. 湖南中医药大学针灸推拿与康复学院，长沙 410208
- Keywords:
Hypoxia-ischemia, brain;
Brain injuries;
Abdominal massage;
Behavioral;
5-HTR1A;
Rats
- From:
International Journal of Traditional Chinese Medicine
2022;44(12):1389-1394
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of abdominal massage on the behavior of rats with neonatal hypoxia-ischemia and its mechanism.Methods:7-day-old SD rats were made as the HIBD model by the classical method of RICE and then the HIBD model rats were divided into the abdominal massage group and model group according to the random number table method, with 12 rats in each group, and 12 rats were selected as the normal group. The abdominal massage group was given abdominal massage 24 hours after the modeling, and the intervention continued for 28 days. Rats in each group underwent a balance beam test on the 7th, 14th, 21st, and 28th day of the intervention. After the intervention, HE staining was applied to observe the morphological structure of the hippocampal CA1 region of the rats; Quantitative Real-time PCR method was used to measure the serotonin receptor (5-HTR1A) in the hippocampus. The expression of cAMP, PKA and CREB in the hippocampus were measured by immunohistochemistry, and the expression of SYP protein was measured by Western blotting.Results:After the intervention, the cells in the hippocampal CA1 area of the model group were diffusely distributed, the number of neurons reduced, and the condition of inflammatory edema appeared; the cells in hippocampal CA1 area of the abdominal massage group were arranged clearly, and the condition of inflammatory edema has significantly improved; on the 21st and 28th day of the intervention, the balance beam test scores in the abdominal massage group significantly decreased ( P<0.05), and the relative expression of 5-HTR1A mRNA (1.18±0.08 vs. 0.77±0.04) in the abdominal massage group significantly increased ( P<0.05). The expression of cAMP (0.32±0.02 vs. 0.31±0.01), PKA (0.32±0.02 vs. 0.29±0.01),CREB (0.31±0.02 vs. 0.28±0.01) and SYP in the abdominal massage group significantly increased ( P<0.05). Conclusion:Abdominal massage could improve the behavior of neonatal hypoxic-ischemic rats, which may play a role on nerve repair by regulating 5-HTR1A/cAMP/PKA signaling pathway.
