Content determination of ginsenoside Rb1, cinnamic acid, paeoniflorin and berberine in Wenxin Formula based on UPLC-MS/MS MRM mode
10.3760/cma.j.cn115398-20220316-00188
- VernacularTitle:基于UPLC-MS/MS多反应监测的温心方人参皂苷Rb1、肉桂酸、芍药苷及小檗碱同时定性定量分析
- Author:
Zongwei WANG
1
;
Xuena LI
;
Chang LIU
;
Guangli YAN
;
Xijun WANG
;
Hui SUN
Author Information
1. 黑龙江中医药大学 教育部经典名方有效性评价及产业化开发工程研究中心 国家中医药管理局中医方证代谢组学研究中心，哈尔滨 150040
- Keywords:
Assay (TCD);
Wenxin Formula;
UPLC-MS/MS;
Qualitative and quantitative analysis;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2022;44(10):1138-1143
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a qualitative and quantitative analysis method of ginsenoside Rb1, cinnamic acid, paeoniflorin and berberine in Wenxin formula based on UPLC-MS/MS MRM model so as to provide a rapid and accurate evaluation method for the research and development of new drugs.Methods:Adopting Waters ACQUITY UPLCTM HSS T3 (2.1 mm×100 mm, 1.8 μm) and gradient elution was performed by mobile methanol-0.03% formic acid water. chromatographic column was used with electrospray ionization source in the scanning mode of multiple reactive ion monitoring (MRM) for ion separation and screening. The retention time and the relative abundance ratio (qualitative ion pairs/quantitative ion pairs) was used for qualitative analysis, while quantitative ion pairs was used for quantitative analysis.Results:The four components tested in Wenxin Formula have been qualitatively detected and showed a good linear relationship, the method showed accuracy, precision, repeatability, and stability, the recovery rate was 96.24%-104.19% and RSD was 1.29%-3.30%. Conclusion:The established method is simple, accurate, rapid and sensitive, which is reliable for the qualitative and quantitative study of the four main components in Wenxin Formula.
