The potential mechanism of Jiajian Xuezheng Decotion in infiltrative gastric cancer through on network pharmacology and proteomics
10.3760/cma.j.cn115398-20210821-00248
- VernacularTitle:基于网络药理学和蛋白质组学探讨加减血癥汤对浸润型胃癌的作用
- Author:
Lihua ZHANG
1
;
Huiqin ZHUO
;
Jingjing HOU
;
Huiping ZHANG
;
Yang ZHOU
;
Jianchun CAI
Author Information
1. 厦门大学附属中山医院胃肠外科，厦门 361004
- Keywords:
Proteomics;
Infiltrative gastric cancer;
Jiajian Xuezheng Decotion;
Active constituent;
Network pharmacology;
Molecular mechanisms of pharmacological actio
- From:
International Journal of Traditional Chinese Medicine
2022;44(10):1150-1156
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the potential mechanism of Jiajian Xuezheng Decotion in infiltrative gastric cancer by network pharmacology and proteomics.Methods:The Traditional Chinese Medicine Systems Pharmacology Database (TCMSP) database was used to find the compounds and their targets of Jiajianxuezhengtang, and the targets of invasive gastric cancer were determined by high performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). The predicted target gene of Jiajian Xuezheng Decotion and the target protein data of infiltrative gastric cancer were analyzed by Venny to obtain the target gene. The target gene set was analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment by the David. The protein interaction network diagram (PPI) was obtained by the String method, displaying the prescription-drug-compound-gene network in Cytoscape software.Results:69 active ingredients and 215 drug targets were screened from Jiajian Xuezheng Decotion; 660 proteins were significantly up-expressed in infiltrative gastric cancer, and 10 drug targets and gene targets were the common targets. There were 10 protein nodes in the PPI network, of which 3 core nodes were CASP3, BCL2L1 and STAT1. The 11 KEGG pathways were significantly enriched such as include PI3K-Akt signaling pathway, p53 signaling pathway, proteoglycan in cancer, apoptosis, Jak-STAT signaling pathway and other pathways.Conclusions:Jiajian Xuezheng Decotion plays an anti-infiltrative gastric cancer effect possibly regulated apoptosis through PI3K-Akt signaling pathway, p53 signaling pathway and Jak-STAT signaling pathway. This study provides a theoretical basis for further research on the mechanism of Jiajian Xuezheng Decotion in the treatment of invasive gastric cancer.
