Mechanism of Acanthopanax senticosus injection in the treatment of ischemic stroke based on UPLC-Q-TOF-MS and network pharmacology
10.3760/cma.j.cn115398-20220120-00232
- VernacularTitle:基于UPLC-Q/TOF-MS整合网络药理学的刺五加注射液治疗缺血性脑卒中作用机制研究
- Author:
Xinghua LI
1
;
Guangli YAN
;
Hongda LIU
;
Lijun FAN
;
Yan YAN
;
Xijun WANG
Author Information
1. 黑龙江中医药大学中医方证代谢组学研究中心，哈尔滨 150040
- Keywords:
Ci Wu Jia Zhu She Ye;
Ischemic stroke;
UPLC-Q-TOF-MS;
Network pharmacology;
Molecular docking simulation;
Molecular mechanisms of pharmacological action (T
- From:
International Journal of Traditional Chinese Medicine
2022;44(8):901-909
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyse the material basis and potential mechanism of Acanthopanax senticosus injection (ASI) in the treatment of ischemic stroke by combining UPLC-Q/TOF-MS and network pharmacology.Methods:The chemical composition of ASI was identified by UPLC-Q/TOF-MS. The Swiss Target Prediction, GeneCards and OMIM databases were used to predict the potential targets for the action of ASI in the treatment of ischemic stroke. The String database and Cytoscape software were used to construct protein interaction network maps, and the Omicshare platform was used to perform gene ontology (GO) and KEGG enrichment analysis. The DockThor platform was used for molecular docking.Results:The analysis of 53 components in ASI was firmly established and used as a basis to obtain 189 related targets of ASI for the treatment of ischemic stroke. Reverse screening showed that 25 components in ASI may be important active components in the treatment of ischemic stroke. Functional enrichment studies found that ASI may mainly regulate PI3K-Akt signaling pathway to treat ischemic stroke.Conclusion:This study preliminarily predicted the mechanism of ASI in the treatment of ischemic stroke may be related to inhibition of inflammation, antioxidant stress, promotion of angiogenesis and protection of nerve cells.
