Study on the chemical composition differences of different parts of Alpinia oxyphylla Miq.based on multivariate statistical analysis
10.3760/cma.j.cn115398-20211227-00288
- VernacularTitle:基于多元统计分析的益智不同部位化学成分差异性研究
- Author:
Wuyi LI
1
;
Meizhou LI
;
Ting CUI
;
Yanzhen LI
;
Yao HUANG
;
Sen HUANG
;
Zhipeng ZHANG
Author Information
1. 广东一方制药有限公司 广东省中药配方颗粒企业重点实验室，佛山 528244
- Keywords:
Alpiniae oxyphyllae fructus;
Medicinal portions;
Fingerprint;
Multivariate statistical analysis
- From:
International Journal of Traditional Chinese Medicine
2022;44(7):776-780
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the Ultra-High Performance Liquid Chromatography (UPLC) characteristic chromatogram of different parts of Alpinia oxyphylla Miq., and to compare different parts of the chemical components based on multivariate statistical analysis. Methods:The UPLC was used to establish the fingerprint of Alpinia oxyphylla Miq. . The chromatograms were matched to generate the UPLC charactersistic chromatogram of different parts. Based on the variance analysis of single factor, combined with the Principal Component Analysis (PCA) ,Cluster Analysis (CA) and the Partial Orthogonal Least Square Discriminant Analysis (OPLS-DA) to analyze the differences of different medicinal parts of Alpinia oxyphylla Miq.. Results:16 common peaks of Alpinia oxyphylla Miq. were demarcated in crude drugs, compared with the medicinal materials of Alpinia oxyphylla Miq., the peak 13 (tectochhrysin) was lost in the decoction pieces, and the shell were missing peak 5 and peak 6. The results of PCA and CA showed that 15 batches of different medicinal parts of Alpinia oxyphylla Miq. can be broadly divided into 3 categories. The OPLS-DA result showed that the value of the peak area of peaks 14 (Nootkatone), 4, 7 and 12 were the main factors affecting the chemical composition of different parts of Alpinia oxyphylla Miq. .Conclusion:The fingerprint determination method established in this study is stable and controllable, which could distinguish the different parts of Alpinia oxyphylla Miq. .
