Research of Shangke Huoxue Decoction based on multi-component TLC identification and content determination
10.3760/cma.j.cn115398-20210923-00295
- VernacularTitle:伤科活血水煎液多组分薄层色谱法鉴别及含量测定
- Author:
Xu ZHOU
1
;
Runkong WANG
;
Mingquan WU
;
Liyang ZHU
;
Wei PENG
;
He TU
Author Information
1. 四川省骨科医院中药研究室，成都 610041
- Keywords:
Chromatography, thin layer;
Chromatography, high pressure liquid;
Shangke Huoxue Decoction;
Active constituent;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2022;44(7):781-785
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the TLC identification method and content determination method of ferulic acid, ligustilide, hydroxysafflor yellow A and paeoniflorin in Shangke Huoxue Decoction for quality evaluation.Methods:Ferulic acid, ligustilide, hydroxysafflor yellow A and paeoniflorin were qualitatively identified by TLC method, and the content was determined by HPLC method. Waters Symmetry ShieldTM RP18 column (4.6 mm×250 mm, 5 μm) was set, the mobile phase consisted of acetonitrile-0.15% phosphoric acid water with gradient elution at a flow of 1.0 ml/min, and the column temperature was 30 ℃.The detection wavelength was 320 nm (33-50 min for ferulic acid, 55-70 min for ligustilide), 403 nm (7-31 min for hydroxysafflor yellow A) and 230 nm (7-31 min for paeoniflorin).Results:The TLC spots were clear. The linear relationships of ferulic acid, ligustilide, and hydroxysafflor yellow A were good in the range of 3.05-48.74 μg, 3.50-26.24 μg, 21.34-213.44 μg. The method was stable, repeatable with good recovery rate.Conclusion:The TLC and HPLC method for the simutanous determination of the four effective components in Shangke Huoxue Decoction were established, and the methods are suitable for the quality evaluation of Shangke Huoxue Decoction.
