Study on HPLC fingerprint of Myristica fragrans and its components identification
10.3760/cma.j.cn115398-20210915-00201
- VernacularTitle:肉豆蔻指纹图谱研究及成分解析
- Author:
Liqing GU
1
;
Jiantong YUAN
;
Songhua HE
;
Qixia NING
Author Information
1. 广西壮族自治区食品药品检验所国家药品监督管理局中药材质量监测与评价重点实验室，南宁 530021
- Keywords:
Myristica fragrans (TCD);
HPLC fingerprint;
Tandem mass spectrometry;
Dehydroisoeugenol;
Quality control
- From:
International Journal of Traditional Chinese Medicine
2022;44(6):661-666
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for quality evaluation of Myristica fragrans Houtt. Methods:The common peak was determined with Dehydroisoeugenol as the reference peak, and the HPLC fingerprint of Myristica fragrans was established; then the common peaks were analyzed by High Resolution Liquid Chromatography-mass Spectrometry (HPLC-MS). The chemical components of the common peaks were identified through the calculation and data retrieval of the primary and secondary mass spectra of the characteristic peaks. Results:The HPLC fingerprint of Myristica fragrans was established, and the similarity degree of the 10 batches of samples was above 0.9 and 11 common peaks were established. According to the results of HPLC-MS, the components of 11 common peaks were identified as follow: Methyl eugenol (peak 1), Licarin A (peak 2), Myristol (peak 3), OdoratisolA (peak 4), 2-(3,4-Dimethoxyphenyl) butynoic acid (peak 5), Malabaricon D (peak 6), 5'-Methoxydehydroisoeugenol (peak 7), Dehydroisoeugenol (peak 8), Malabaricone C (peak 9), 4-Methoxy-6-{(2S,3S)-7-methoxy-3-methyl-5-[(1E)-1-propen-1-yl]-2,3-dihydro-1- benzofuran-2-yl}-1,3-benzodioxole (peak 10) and Licarin B (peak 11). Conclusions:The quality of Myristica fragrans could be evaluate with HPLC fingerprint method. HPLC-MS was used to analyze the chemical composition of complex components, which will provide reference for the identification and analysis of chemical components of the extracts and preparations of Traditional Chinese Medicine.
