Research of esomeprazole on inhibiting proliferation and chemosensitization of breast cancer cells
10.3760/cma.j.cn115396-20220207-00038
- VernacularTitle:艾司奥美拉唑抑制乳腺癌细胞增殖及化疗增敏效果的研究
- Author:
Guanqun LIU
1
;
Lingyue DONG
;
Zhihua LONG
;
Qing XU
Author Information
1. 首都医科大学大兴教学医院胸外血管外科，北京 102600
- Keywords:
Breast neoplasms;
Cell proliferation;
Cell cycle;
Esomeprazole;
Chemosensitivity
- From:
International Journal of Surgery
2022;49(10):689-693,C4
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effect of esomeprazole on proliferation and chemosensitizing effect of breast cancer cells.Methods:Human MBA-MD-231, MCF-7 breast cancer cell line and human Huh7 liver cancer cell line were cultured by conventional methods; cells were treated with different concentrations of esomeprazole, and CCK8 kit was used to detect the proliferation of different tumor cells after stimulation. Cells were treated with different concentrations of esomeprazole, and the effects of esomeprazole on cell cycle of different cells were analyzed by flow cytometry. Cells were treated with different concentrations of paclitaxel and epirubicin combined with esomeprazole, and CCK8 kit was used to detect the proliferation of different tumor cells after stimulation. Measurement data were expressed as mean ± standard deviation ( ± s), and analysis of variance was used for comparison among multiple groups. Results:CCK8 results showed that esomeprazole could inhibit the proliferation of MBA-MD-231 cells, MCF-7 cells and Huh7 cells in a dose-dependent manner. Flow cytometry results showed that cells in G 0/G 1 phase were significantly increased by esomeprazole treatment. Esomeprazole can enhance the inhibitory effect of paclitaxel and epirubicin on the proliferation of MBA-MD-231 cells and MCF-7 cells, and improve the chemosensitivity. Conclusion:Esomeprazole blocks breast cancer cell MBA-MD-231, MCF-7 and liver cancer cell Huh7 in G 0/G 1 phase, thereby inhibiting cell proliferation. Esomeprazole can enhance the inhibitory effect of chemotherapeutic drugs on the proliferation of MBA-MD-231 and MCF-7 cells.
