Bacteria resistance and cytotoxicity of copper loaded coatings on ureteral stent
10.3760/cma.j.cn121382-20210907-00203
- VernacularTitle:输尿管支架表面载铜涂层抗菌性能及生物相容性研究
- Author:
Hongwei LI
1
;
Jianzhong LI
;
Jing ZHAO
;
Lianhui FAN
Author Information
1. 中国人民解放军北部战区总医院泌尿外科，沈阳 110083
- Keywords:
Ureteral stent;
Coating;
Copper;
Anti-bacteria;
Cytotoxicity
- From:
International Journal of Biomedical Engineering
2022;45(2):104-111
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the antimicrobial properties of copper-loaded coatings on the surface of ureteral stents and their biocompatibility in order to determine the most suitable level of copper loading.Methods:Copper-loaded PDA coatings with different copper contents were constructed on the surface of polyurethane (PU) stents using polydopamine (PDA) and dimethylaminomethylborane (DMAB). The antibacterial property of the coating against Escherichia coli and Staphylococcus aureus was investigated by the plate counting method. The bacterial adhesion on the coating surface was studied by scanning electron microscopy. Using the live/dead evaluation, microbes were stained and observed by a fluorescence microscope. The biocompatibility of the copper-loaded coatings was detected by a cell proliferation assay incubated with L929 cells.Results:The antibacterial rates of the copper-loaded samples exceeded 90% after incubation with E. coli and S. aureus for 24 h, respectively, and the antibacterial performance increased with the increase of copper content in the coating. The amount of bacteria adhered to the surface of the copper-loaded samples was significantly lower, and most of them were dead bacteria. When the copper content in the coating preparation solution used was 0.25~1 g/L, the cell proliferation rate on the surface of the copper-loaded coating was higher than 80% and the material was not cytotoxic.Conclusions:A copper-loaded PDA coating with excellent antibacterial properties and good biocompatibility can be prepared with a copper content of 1 g/L in the coating preparation solution, forming a potential solution for the preparation of ureteral stent coatings.
