The activation of hippocampal pericytes and glial scar formation in rats with status epilepticus
10.3760/cma.j.issn.1673-4408.2022.07.013
- VernacularTitle:癫痫持续状态大鼠海马周细胞活化及胶质瘢痕形成的实验研究
- Author:
Danrui DING
1
;
Jie LEI
;
Luxi RAN
;
Hua WANG
Author Information
1. 中国医科大学附属盛京医院小儿神经内科，沈阳 110004
- Keywords:
Rats;
Pericyte;
Glial cell;
Glial scar;
Status epilepticus
- From:
International Journal of Pediatrics
2022;49(7):488-492,F3
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the perivascular activation of reactive pericytes after status epilepticus(SE), and the relationship between pericytes and glial cells in proliferation and function.Methods:Eighty rats were randomly divided into control group( n=16) and model group( n=64, 16 for each group in SE1d, SE3d, SE7d, SE28d). The SE model was induced by intraperitoneal injection of lithium chloride and pilocarpine, and hematoxylin-eosin staining was performed on brain tissue sections to observe basic pathological changes.Use immunohistochemistry and Western blot to detect(neuron-glial antigen 2, NG2) expression, and use immunofluorescence technology to double stain NG2 and(glial fibrillary acidic protein, GFAP) to observe their relationship. Results:In the model group, the neurons were arranged disorderly, losing the ribbon structure, and the neurons appeared degeneration and necrosis.It was observed that the nuclei of the neurons were blurred, and the cytoplasm was agglomerated.There were more glial cells proliferation.Compared with the control group, it was found in model group that NG2 showed a dynamic high expression after SE( P<0.05). The number of pericytes increased significantly, reaching a peak at 7d, and the results of Western blot were consistent with the results of histochemistry( P<0.05). The aggregation of glial cells were induced in the surrounding area, and pericytes participated in the signal transduction of glial cells. Conclusion:Pericytes can induce the aggregation of glial cells and participate in the repairment in the form of glial scars after SE brain injury.
