Etiological identification and phylogenetic analysis of a clustered epidemic caused by norovirus on a patrol boat
10.19428/j.cnki.sjpm.2022.21945
- VernacularTitle:某巡逻船聚集性诺如病毒疫情的病原基因特征分析
- Author:
Jiwei SHU
1
;
Linfu GUAN
1
;
Tongjie ZHANG
1
;
Yi REN
1
;
Ling YE
1
;
Ni XU
2
;
Rong WANG
3
;
Maowen HUANG
4
Author Information
1. Daishan Center for Disease Control and Prevention， Zhoushan， Zhejiang 316299， China
2. Daishan First People's Hospital， Zhoushan， Zhejiang 316261， China
3. Ningbo Center for Disease Control and Prevention， Ningbo， Zhejiang 315099， China
4. Ningbo Beilun District People's Hospital， Ningbo， Zhejiang 315826， China
- Publication Type:Journal Article
- Keywords:
recombinant;
GⅠ.6 ［P11］;
etiological identification;
genotyping
- From:
Shanghai Journal of Preventive Medicine
2022;34(11):1112-1117
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo determine the pathogen and phylogenetic characteristics of an uncommon outbreak of recombinant norovirus infection in Daishan County in February 2022. MethodsFluorescence quantitative PCR was used to detect the norovirus in the eight anal swabs collected in the outbreak. In the positive samples， reverse transcription PCR were used to amplify the norovirus. Norovirus sequences were characterized by MEGA7 and Simplot. ResultsNorovirus GⅠ was identified in all eight anal samples. It was further determined to be recombinant norovirus GⅠ.6 ［P11］， with the recombination site at the ORF1-ORF2 junction. The sequence had the highest nucleotide identity （98.75%） to a GⅠ.6［P11］ strain collected in 2018 （GenBank accession number MT357995）. ConclusionAccording to the etiological identification and phylogenetic analysis， this outbreak is confirmed to be caused by the uncommon recombinant norovirus GⅠ.6 ［P11］ in China.
