Epidemiological investigation on a visceral leishmaniasis case in Zhengzhou City of Henan Province
10.16250/j.32.1374.2022048
- VernacularTitle:郑州市1例内脏利什曼病病例流行病学调查
- Author:
Cheng-yun YANG
1
;
Xiao-ming ZHAO
2
;
De-ling LU
1
;
Yue-quan ZHANG
3
;
Jian-hua QIAN
4
;
Xiao WANG
5
;
Su-hua LI
1
;
Zhi-quan HE
1
;
Dan QIAN
1
;
Ying LIU
1
;
Peng-hui JI
1
;
Rui-min ZHOU
1
;
Hong-wei ZHANG
1
Author Information
1. Henan Provincial Center for Disease Control and Prevention, Henan Provincial Key Laboratory for Pathogenic Microorganisms of Infectious Diseases, Zhengzhou, Henan 450016, China
2. The First Affiliated Hospital of Zhengzhou University, Henan Province, China
3. Zhengzhou Center for Disease Control and Prevention, Henan Province, China
4. Xinmi Center for Disease Control and Prevention, Henan Province, China
5. Erqi District Center for Disease Control and Prevention, Zhengzhou City, Henan Province, China
- Publication Type:Journal Article
- Keywords:
Visceral leishmaniasis;
Sandfly;
Leishmania infantum;
Epidemiological investigation;
Zhengzhou City
- From:
Chinese Journal of Schistosomiasis Control
2022;34(6):635-638
- CountryChina
- Language:Chinese
-
Abstract:
Objective To perform an epidemiological investigation on a case with visceral leishmaniasis in Zhengzhou City, Henan Province, and to identify the source of infection, so as to illustrate the transmission chain and assess the risk of local leishmaniasis transmission. Methods The medical data were collected from a case with visceral leishmaniasis in Zhengzhou City, and the patient’s bone marrow smears were detected by microscopy. Serum anti-Leishmania antibody test and PCR assay were performed among high-risk residents and all dogs in the village where the patient lived. Sandflies were captured using light traps and artificial traps, and the captured female Phlebotomus chinensis was subjected to PCR assay. The internal transcribed spacer 1 (ITS1) gene was amplified with a nested PCR assay using the genomic DNA extracted from visceral leishmaniasis patients, positive dogs and sandflies, and the sequences were aligned with those download from NCBI. In addition, a phylogenetic tree was created based on the ITS1 gene. Results The visceral leishmaniasis patient had recurrent irregular fever, reduced complete blood counts, low hemoglobin, and a large number of Leishmania amastigotes in bone marrow smears, and was therefore diagnosed as visceral leishmaniasis. Both rk39 rapid diagnostic test and PCR assay tested negative among 324 residents living neighboring the patient’s residence, while 21.39% (43/201) dogs were positive for rk39 rapid diagnostic test and 13.93% (28/201) positive for PCR assay. There were 17 female Ph. chinensis tested positive for Leishmania (0.82%) by PCR assay, and the ITS gene sequences from visceral leishmaniasis patients, positive dogs and sandflies shared a 100% homology with L. infantum. The Leishmania species was therefore characterized as L. infantum. Conclusions L. infantum infection occurs in visceral leishmaniasis patients, dogs and sandflies in Zhengzhou City, indicating a complete transmission chain and a high transmission risk of visceral leishmaniasis by L. infantum. Intensified control measures are required to prevent local transmission of leishmaniasis in Zhengzhou City.
