Bacterial community diversity in Dermatophagoides farinae using high-throughput sequencing
10.16250/j.32.1374.2022105
- VernacularTitle:基于高通量测序的粉尘螨体内细菌多样性研究
- Author:
Xiao-qian ZHOU
1
;
Jie MA
2
;
Rui-yi WANG
3
;
Ruo-hang WANG
2
;
Yi-qiang WU
2
;
Xin-yan YANG
2
;
Yu-juan CHEN
3
;
Xiao-niu TANG
1
;
En-tao SUN
2
Author Information
1. Department of Medical Parasitology, Wannan Medical College, Wuhu, Anhui 241002, China
2. Department of Health Inspection and Quarantine, Wannan Medical College, Wuhu, Anhui 241002, China
3. Department of Environmental Hygiene and Occupational Health, School of Public Health, Wannan Medical College, China
- Publication Type:Journal Article
- Keywords:
Dermatophagoides farinae;
Bacterial diversity;
16S ribosomal RNA;
High-throughput sequencing
- From:
Chinese Journal of Schistosomiasis Control
2022;34(6):630-634
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the bacterial community diversity in Dermatophagoides farinae. Methods Laboratory-cultured D. farinae was collected, and the composition of microbial communities was determined by sequence analyses of the V4 region in the bacterial 16S ribosomal RNA (16S rRNA) gene on an Illumina PE250 high-throughput sequencing platform. Following quality control and filtering of the raw sequence files, valid reads were obtained and subjected to operational taxonomic units (OTU) clustering and analysis of the composition of microbial communities and alpha diversity index using the Usearch software, Silva database, and Mothur software. Results A total of 187 616 valid reads were obtained, and 469 OTUs were clustered based on a sequence similarity of more than 97%. OTU annotation showed that the bacteria in D. farinae belonged to 26 phyla, 43 classes, 100 orders, 167 families and 284 genera. The bacteria in D. farinae were mainly annotated to five phyla of Proteobacteria, Firmicutes, Bacteroidota, Actinobacteriota, and Acidobacteriota, with Proteobacteria as the dominant phylum, and mainly annotated to five dominant genera of Ralstonia, norank-f-Mitochondria, Staphylococcus and Sphingomonas, with Wolbachia identified in the non-dominant genus. Conclusions A high diversity is identified in the composition of the bacterial community in D. farinae, and there are differences in bacterial community diversity and abundance among D. farinae.
