Inflammation Response Related Gene Expression Profile after Injury of Rubrospinal Tract

VernacularTitle:大鼠颈段红核脊髓束损伤后炎症反应相关基因表达谱
Author: Chao WANG ; Tiansheng SUN ; Jingcheng JIANG ; Chaoqun YE
Publication Type:Journal Article
Keywords: spinal cord injury, rubrospinal tract, gene chip, rat
From: Chinese Journal of Rehabilitation Theory and Practice 2011;17(4):337-339
CountryChina
Language:Chinese
Abstract: ObjectiveTo investigate the characteristic changes of expression of the genes related to inflammation response after injury of rubrospinal tract（RST）. Methods18 Sprague Dawley（SD）female rats were randomly divided into 2 groups: RST injury group (n=9) and Sham group (n=9). RST injury models were established, and the rats were killed 24 hours after injury. 5 mm length spinal cord was harvested from the epicenter and total RNA was extracted. Affymetrics Gene Chips for rats, representing 28000 genes, were used for mRNA expression profiling.Results153 transcripts were observed to differ (2.0 fold; 136 up-regulated and 17 down-regulated) after injury of RST, compared with sham group. Most of genes related to inflammation response were up-regulated (except Scn9α). 8 genes related to Toll-like receptor signaling pathway were also up-regulated.ConclusionSignificant changes related to inflammation response occur in acute phase after injury of RST.