Preparation and anti-Vibrio vulnificus hemolysin A of macrophage membrane hybrid liposome
10.12206/j.issn.2097-2024.202207001
- VernacularTitle:巨噬细胞膜杂合脂质体的制备及抗创伤弧菌溶血素A的作用研究
- Author:
Yu DAI
1
;
Lingyi GUO
1
;
Hongbo WANG
1
;
Kangqing BIAN
1
;
Yuan YU
1
Author Information
1. Department of Pharmaceutical Science, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Keywords:
Vibrio vulnificus;
hemolysin A;
biomimetic nanocarrier;
bacterial infections
- From:
Journal of Pharmaceutical Practice
2023;41(1):26-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a biomimetic nano carrier macrophage membrane hybrid liposome by heterozygous macrophage membrane and liposome, which could be used for the clearance and toxicity inhibition of Vibrio vulnificus hemolysin A (VvhA). Methods Macrophage membrane was extracted and hybridized with liposome by thin-film evaporation combined extrusion method. The hybridized liposome of macrophage membrane was constructed and characterized. The in vitro detoxification ability of the hybridized vector was evaluated by hemolysis test and cytotoxicity test. The detoxification ability of the vector was evaluated by mouse skin infection model. Results Anti toxoid studies in vivo and in vitro showed that the anti-hemolysis rate of macrophage membrane heterozygous liposomes in vitro reached 97.03%, which could effectively inhibit the skin ulceration in subcutaneous infected mice and make the survival rate of abdominal infected mice reach 80%. Conclusion The constructed macrophage membrane hybrid liposome had high detoxification ability, which could provide a potential solution and research basis for the prevention and treatment of Vibrio vulnificus infection.
