Improvement effect of active ingredients of Anemarrhena asphodeloides on sepsis-induced myocardial injury model mice
- VernacularTitle:知母活性成分对脓毒症性心肌损伤模型小鼠的改善作用
- Author:
Qian LI
1
,
2
;
Xiangming CHEN
1
,
2
;
Xinxin CHANG
1
,
2
;
Wenhua CUI
1
;
Yang LI
1
,
2
;
Zijing XUE
1
,
2
;
Yuguang ZHENG
1
,
2
,
3
;
Junna SONG
1
Author Information
1. College of Pharmacy，Hebei University of Chinese Medicine/Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province，Shijiazhuang 050091，China
2. International Joint Research Center on Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province，Shijiazhuang 050091，China
3. Hebei Chemical and Pharmaceutical College，Shijiazhuang 050026，China
- Publication Type:Journal Article
- Keywords:
Anemarrhenae asphodeloides;
total xanthone
- From:
China Pharmacy
2023;34(1):29-33
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To provide reference for clarifying improvement effects of Anemarrhena asphodeloides on sepsis- induced myocardial injury and potential material basis. METHODS Water extract of A. asphodeloides was extracted by thermal reflux method. Total xanthone and total saponins in A. asphodeloides were separated by macroporous adsorption resin. The mice model of sepsis-induced myocardial injury was established by intraperitoneal injection of lipopolysaccharide. The effects of the location of three extraction fractions and the monomers of A. asphodeloides as mangiferin， timosaponin AⅢ and timosaponin BⅡ on the survival rate of the model mice were explored. HE staining was used to observe the effects of mangiferin， timosaponin AⅢ and timosaponin BⅡ on myocardial morphology in model mice. The effects of mangiferin on mRNA expressions of inflammatory cytokines [interleukin-6 （IL-6）， IL-1β and tumor necrosis factor-α （TNF-α）] and the level of reactive oxygen species （ROS） in myocardial tissue of model mice were detected. RESULTS Compared with the model group， the survival rate of mice in the intervention group of total xanthone， total saponins and water extract was increased to different extents， especially total xanthone fraction. Mangiferin， timosaponin AⅢ and timosaponin BⅡcould improve the degree of myocardial cell swelling and muscle bundle arrangement disorder in model mice， especially mangiferin. Compared with model group， mRNA expressions of IL-6， IL- 1β and TNF- α， ROS level in myocardium of mice after mangiferin intervention were decreased to different extents. CONCLUSIONS The different extraction fractions of A. asphodeloides can improve survival rate of mice with sepsis-induced myocardial injury， especially total xanthone fraction. Mangiferin is the best among the three monomers of A. asphodeloides to improve sepsis-induced myocardial injury， which may play a role in anti-sepsis myocardial injury by anti-inflammation and antioxidantion.
