Genome sizes of four important medicinal species in Kadsura by flow cytometry
10.1016/j.chmed.2021.05.002
- Author:
Jing XU
1
;
Xue-ping WEI
1
;
Jiu-shi LIU
1
;
Yao-dong QI
1
;
Ben-gang ZHANG
1
;
Hai-tao LIU
1
;
Pei-gen XIAO
1
;
Xue-ping WEI
2
;
Jiu-shi LIU
2
;
Yao-dong QI
2
;
Ben-gang ZHANG
2
;
Hai-tao LIU
2
;
Pei-gen XIAO
2
Author Information
1. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College
2. Engineering Research Center of Tradition Chinese Medicine Resource, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College
- Publication Type:Journal Article
- Keywords:
Dianjixueteng;
flow cytometry;
genome size;
Kadsura interior A. C. Smith
- From:
Chinese Herbal Medicines
2021;13(3):416-420
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Dianjixueteng is a geoherb in Yunnan Province, the source plant of which is Kadsura interior. However, the formation of this geoherb is not clear in genetic mechanism, in which genome size is the first step that should be known on the genomic level. In this study we aimed to estimate the genome sizes of source plants of K. interior and three related herbs K. heteroclita, K. longipedunculata, and K. coccinea by flow cytometry (FCM) and make a comparison. Methods: The genome sizes of K. interior, K. heteroclita, K. longipedunculata and K. coccinea, i.e., the source plants of Dianjixueteng and its relative medicinal materials, were estimated by FCM. The nuclei of K. interior were isolated using modified LB01 buffer, for the rest species, by the Galbraith's buffer. Results: The genome sizes of K. interior, K. heteroclita, K. longipedunculata, and K. coccinea were 7.36, 7.12, 7.01, and 5.15 pg/1C, respectively. Genome size of K. interior had no significant variation with those of K. heteroclita and K. longipedunculata (P = 0.296), which was significantly larger than that of K. coccinea. Conclusion: Genome size can not distinguish K. interior from K. heteroclita and K. longipedunculata, but could distinguish them from K. coccinea, which lays the foundation for future studies on genetic mechanism of the geoherb formation.
