High water-soluble curcuminoids-rich extract regulates osteogenic differentiation of MC3T3-E1 cells: Involvement of Wnt/β-catenin and BMP signaling pathway
10.1016/j.chmed.2021.01.003
- Author:
Yutthana PENGJAM
1
;
Jakkapong INCHAI
1
;
Amornkan NUMIT
1
;
Nurul SYAZWANI
2
;
Pharkphoom PANICHAYUPAKARANANT
2
;
Thanintorn YODTHONG
3
;
Thanawat PITAKPORNPREECHA
3
Author Information
1. Faculty of Medical Technology, Prince of Songkla University
2. Faculty of Pharmaceutical Sciences, Prince of Songkla University
3. Faculty of Science, Prince of Songkla University
- Publication Type:Journal Article
- Keywords:
BMP signaling;
curcuminoid;
osteoporosis;
solid dispersion;
water soluble;
Wnt/β-catenin
- From:
Chinese Herbal Medicines
2021;13(4):534-540
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The present study aimed to evaluate the effect of a high water-soluble curcuminoids-rich extract (CRE) in a solid dispersion form (CRE-SD) using polyvinylpyrrolidone K30 on osteogenic induction of MC3T3-E1 cells. Methods: CRE was pre-purified using a microwave assisted extraction couple with a Diaion® HP-20 column chromatography. The osteoblastic cell proliferation and differentiation potentials of CRE-SD in MC3T3-E1 cells were tested by cell viability, alkaline phosphatase (ALP) activity, and Alizarin red S activity assays. The mRNA expressions of osteoblast-specific genes and underline mechanisms were assessed by a real time PCR and western blot analysis. Results: CRE-SD 50 µg/mL increased alkaline phosphatase (ALP) activity, an early differentiation marker of osteoblasts in both MC3T3-E1 cells and non-osteogenic mouse pluripotent cell line, C3H10T1/2, indicating the action of CRE-SD was not cell-type specific. Alizarin red S activity showed a significant amount of calcium deposition in cells treated with CRE-SD. CRE-SD also upregulated the mRNA expression levels of transcription factors that favor osteoblast differentiation including Bmp-2, Runx2 and Collagen 1a, in a dose dependent manner. Western blot analysis revealed that noggin attenuated CRE-SD-promoted expressions of Bmp-2 and Runx2 proteins. siRNA mediated blocking of Wnt/β-catenin signaling pathway also annulled the influence of CRE-SD, indicating Wnt/β-catenin dependent activity. Inhibition of the different signaling pathways abolished the influence of CRE-SD on ALP activity, confirming that CRE-SD induced MC3T3-E1 cells into osteoblasts through Wnt/β-catenin and BMP signaling pathway. Conclusion: These results collectively demonstrate that CRE-SD may be a potential therapeutic agent for the treatment of osteoporosis.
