6,6′-Bieckol induces apoptosis and suppresses TGF-β-induced epithelial-mesenchymal transition in non-small lung cancer cells
10.1016/j.chmed.2021.05.005
- Author:
Yongxin LI
1
;
Man LIU
1
;
Kunlong YANG
1
;
Jun TIAN
1
Author Information
1. Department of Biomedicine and Food Science, School of Life Science, Jiangsu Normal University
- Publication Type:Journal Article
- Keywords:
6,6′-bieckol;
apoptosis;
epithelial-mesenchymal transition;
migration;
non-small cell lung cancer;
phlorotannin;
traditional Chinese medicine
- From:
Chinese Herbal Medicines
2022;14(2):254-262
- CountryChina
- Language:Chinese
-
Abstract:
Objective: In this study, the aim was to investigate the inhibitory effect of 6,6′-bieckol on the migration and epithelial-mesenchymal transition (EMT) of non-small cell lung cancer (NSCLC) cells, and explore its potential molecular mechanisms. Methods: Cell migration was measured using a CCK8, wound healing, and transwell migration assay. Apoptosis was determined using an Annexin V/propidium iodide staining. Western blotting and immunofluorescence were used to examine the expression level of apoptosis-related proteins and EMT marker proteins. Results: The results showed that 6,6′-bieckol inhibited migration and induced apoptosis of NSCLC cells. Furthermore, 6,6′-bieckol had significantly up-regulated the E-cadherin and down-regulated Snail1 and Twist1 transcriptional levels. 6,6′-Bieckol might inhibit TGF-β-induced EMT by down-regulating Snail1 and Twist1 and up-regulating E-cadherin in lung cancer cells. Conclusion: It is suggested that 6,6′-bieckol has the potential to be developed as a therapeutic candidate for lung cancer.
