Functional and binding studies of gallic acid showing platelet aggregation inhibitory effect as a thrombin inhibitor

Author: Yuxin ZHANG ¹ ; Binan LU ¹ ; Hongjuan NIU ¹ ; Lu FAN ¹ ; Zongran PANG ¹ ; Xing WANG ² ; Yanbin GAO ² ; Yatong LI ² ; Yanling ZHANG ³ ; Yanjiang QIAO ³
Author Information

1. Key Laboratory of Ethnomedicine of Ministry of Education, School of Pharmacy, Minzu University of China
2. Beijing Key Lab of Traditional Chinese Medicine Collateral Disease Theory Research, School of Traditional Chinese Medicine, Capital Medical University
3. Key Laboratory of TCM-Information Engineer of State Administration of TCM, School of Chinese Materia Medica, Beijing University of Chinese Medicine
Publication Type:Journal Article
Keywords: gallic acid; molecular dynamics; natural thrombin inhibitor; platelet aggregation; surface plasmon resonance
From: Chinese Herbal Medicines 2022;14(2):303-309
CountryChina
Language:Chinese
Abstract: Objective: This study was devoted to identifying natural thrombin inhibitors from traditional Chinese medicine (TCM) and evaluating its biological activity in vitro and binding characteristics. Methods: A combination strategy containing molecular docking, thrombin inhibition assay, surface plasmon resonance (SPR) and molecular dynamics simulation were applied to verify the study result. Results: Gallic acid was confirmed as a direct thrombin inhibitor with IC