Tea and Citrus maxima complex induces apoptosis of human liver cancer cells via PI3K/AKT/mTOR pathway in vitro
10.1016/j.chmed.2021.09.015
- Author:
Shuai WEN
1
;
Junxi CAO
1
;
Zhigang LI
1
;
Wenji ZHANG
1
;
Ruohong CHEN
1
;
Qiuhua LI
1
;
Xingfei LAI
1
;
Lingli SUN
1
;
Shili SUN
1
;
Ran AN
2
;
Dongli LI
2
;
Dongli LI
3
Author Information
1. Guangdong Key Laboratory of Tea Resources Innovation & Utilization, Tea Research Institute, Guangdong Academy of Agricultural Sciences
2. School of Biotechnology and Health Sciences, Wuyi University
3. International Healthcare Innovation Institute (Jiangmen)
- Publication Type:Journal Article
- Keywords:
apoptosis;
Citrus maxima cv. Tomentosa;
liver cancer;
signaling pathway;
tea
- From:
Chinese Herbal Medicines
2022;14(3):449-458
- CountryChina
- Language:Chinese
-
Abstract:
Objective: In this study, black tea and Citrus maxima (BT-CM), yellow tea and C. maxima (YT-CM), green tea and C. maxima (GT-CM) as subjects, the active ingredient content and antioxidant activity of three tea and C. maxima (T-CM) were analyzed. The effects of three T-CMs on apoptosis of liver cells in vitro and its mechanism were further explored. Methods: National standard method and HPLC were used for active ingredient analysis. MTT, cell flow cytometry and Western blot were used to analyze the effects of three T-CMs on cell proliferation, apoptosis, and its underlying molecular mechanism. Results: The content of tea polyphenols, free amino acids, ratio of polyphenols and amino acids, ester catechins, non-ester catechins and caffeine in YT-CM and GT-CM was significantly higher than that of BT-CM. The in vitro antioxidant capacity of YT-CM and GT-CM was also significantly stronger than that of BT-CM. Three T-CMs had the effects of inhibiting proliferation, arresting cell cycle and inducing apoptosis in HepG2 and Bel7402 cells, especially YT-CM and GT-CM. Western blot analysis showed three T-CMs activated PI3K/AKT/mTOR signaling pathway and regulated the expression levels of apoptosis-related proteins Bax, Bcl-2 and Caspase-3/9. YT-CM and GT-CM had better ability to change the signal pathway than BT-CM. Conclusion: In short, T-CMs, which combined different degrees of fermentation tea with C. maxima, were rich in nutrients and biologically active substances. T-CMs, especially YT-CM and GT-CM, are healthy drinks that help to prevent and treat liver cancer.
