Reduning Injection prevents carrageenan-induced inflammation in rats by serum and urine metabolomics analysis

Xia Gao; Jiajia Wang; Xialin Chen; Shanli Wang; Chaojie Huang; Quanchang Zhang; Liang Cao; Zhenzhong Wang; Wei Xiao; Xia Gao; Jiajia Wang; Xialin Chen; Shanli Wang; Chaojie Huang; Quanchang Zhang; Liang Cao; Zhenzhong Wang; Wei Xiao; Xia Gao; Jiajia Wang; Xialin Chen; Shanli Wang; Chaojie Huang; Quanchang Zhang; Liang Cao; Zhenzhong Wang; Wei Xiao; Shanli Wang; Chaojie Huang

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Reduning Injection prevents carrageenan-induced inflammation in rats by serum and urine metabolomics analysis

Author: Xia GAO ¹ ; Jiajia WANG ¹ ; Xialin CHEN ¹ ; Shanli WANG ¹ ; Chaojie HUANG ¹ ; Quanchang ZHANG ¹ ; Liang CAO ¹ ; Zhenzhong WANG ¹ ; Wei XIAO ¹ ; Xia GAO ² ; Jiajia WANG ² ; Xialin CHEN ² ; Shanli WANG ² ; Chaojie HUANG ² ; Quanchang ZHANG ² ; Liang CAO ² ; Zhenzhong WANG ² ; Wei XIAO ² ; Xia GAO ³ ; Jiajia WANG ³ ; Xialin CHEN ³ ; Shanli WANG ³ ; Chaojie HUANG ³ ; Quanchang ZHANG ³ ; Liang CAO ³ ; Zhenzhong WANG ³ ; Wei XIAO ³ ; Shanli WANG ⁴ ; Chaojie HUANG ⁴
Author Information

1. Jiangsu Kanion Modern Chinese Medicine Institute
2. State Key Laboratory of Pharmaceutical New-Tech for Chinese Medicine, Jiangsu Kanion Pharmaceutical Co., Ltd.
3. National Enterprise Technology Center, National Post-doctoral Research Workstation, Jiangsu Enterprise Academician Workstation
4. China Pharmaceutical University
Publication Type:Journal Article
Keywords: anti-inflammatory effect; metabolomics; Reduning Injection; UPLC-Q-TOF/MS
From: Chinese Herbal Medicines 2022;14(4):583-591
CountryChina
Language:Chinese
Abstract: Objective: To elucidate the anti-inflammatory mechanism of Reduning Injection (RDN) by analyzing the potential biomarkers and metabolic pathways of the carrageenan-induced inflammatory model from the overall metabolic level. Methods: Rat inflammatory model was established by carrageenan. UPLC-Q-TOF/MS was used to detect and analyze changes of endogenous metabolites in the serum and urine of carrageenan-induced inflammatory rats. Combined with multivariate analysis and databases analysis, inflammatory-related potential biomarkers were screened and identified to analyze possible metabolic pathways. The reliability and biological significance of these biomarkers was verified by metabolic network analysis and correlation analysis with pharmacodynamic indicators. Results: A total of 16 potential biomarkers were screened and identified by multivariate analysis and metabolite databases, among which 13 species could be adjusted by RDN. The metabolism pathway analysis revealed that histidine metabolism, sphingolipid metabolism, and tyrosine metabolism were greatly disturbed. Their biomarkers involved urocanic acid, sphingosine, and norepinephrine, all of which showed a callback trend after RDN treatment. The three biomarkers had a certain correlation with some known inflammatory-related small molecules (histamine, arachidonic acid, Leukotriene B4, and PGE