Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis
10.1016/j.chmed.2021.03.002
- Author:
Xingyan ZHANG
1
;
Huling LI
1
;
Wenjing ZHAO
1
;
Xin LI
1
;
Fujun ZHOU
1
;
Yutong ZHOU
1
;
Tao CUI
1
;
Yuli WANG
1
;
Changxiao LIU
1
;
Xiaoyan LIAN
2
;
Wenjing ZHAO
3
;
Fujun ZHOU
4
;
Tao CUI
4
;
Changxiao LIU
4
;
Yuli WANG
5
;
Changxiao LIU
6
;
Yuli WANG
7
;
Yuli WANG
8
Author Information
1. Key Laboratory of Quality-marker of Traditional Chinese Medicines, Tianjin Institute of Pharmaceutical Research Co., Ltd.
2. Center for Drug Evaluation, National Medical Products Administration
3. Department of Pharmacology, Tianjin Medical University
4. State Key Laboratory of Drug Delivery Technology and Pharmacokinetics, Tianjin Institute of Pharmaceutical Research Co., Ltd.
5. Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemistry Engineering and Technology, Tianjin University
6. Research Unit for Drug Metabolism, Chinese Academy of Medical Sciences
7. Pharmaceuticai Armaceuticai DA REN TANG Group Corporation Limited Traditional Chinese Pharmacy Researchinstitute
8. Tianjin Key Laboratory of Quality Control in Chinese Medicine
- Publication Type:Journal Article
- Keywords:
MAPK signal pathway;
molecular docking;
pro-inflammatory cytokines;
taxifolin
- From:
Chinese Herbal Medicines
2022;14(4):554-562
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Taxifolin is a natural flavonoid compound that can be isolated from onions, grapes, oranges and grapefruit. It also acts as a medicine food homology with extraordinary antioxidant and anti-inflammatory activity. This study aims to explain the protective effects and potential mechanisms of taxifolin against inflammatory reaction. Methods: Levels of interleukin (IL)-6, IL-1β and intracellular reactive oxygen species (ROS) were assessed in different time after the treatment of taxifolin in RAW264.7 cells induced by lipopolysaccharide (LPS). Subsequently, the mRNA and protein levels of inducible nitric oxide synthase (iNOS), vascular endothelial growth factor (VEGF), cyclooxygenase (COX)-2, tumor necrosis factor (TNF)-α and the phosphorylation expression levels of the MAPK signal pathway were also evaluated. A silico analysis was used to explain the binding situation for the investigation of taxifolin and MAPK signal pathway. And then MAPK inhibitors were used to reveal the expression level of iNOS, VEGF, COX-2 and TNF-α in RAW264.7 cells. Results: It was demonstrated that cell inflammatory damage induced by LPS was significantly alleviated after the treatment of taxifolin. Then, the mRNA and protein levels of iNOS, VEGF, COX-2 and TNF-α were reduced and the phosphorylation expression levels of the MAPK signal pathway were down-regulated remarkably as well. In silico analysis, taxifolin could form a relatively stable combination with MAPK signal pathway. MAPK inhibitors showed increasing or decreasing effect in the mRNA levels of iNOS, VEGF, COX-2 and TNF-α, which suggesting that taxifolin down-regulated iNOS, VEGF, COX-2 and TNF-α expressions were not entirely through the MAPK pathway. Conclusion: This finding demonstrated that taxifolin improved the inflammatory responses that partly involved in the phosphorylation expression level of MAPK signal pathway in RAW264.7 cells exposed to acute stress.
