Comparative study on brain pharmacokinetics of Buyang Huanwu Decoction in normal and cerebral ischemia rats using brain microdialysis combined with LC-MS/MS
10.1016/j.chmed.2022.03.007
- Author:
Wanyi LIU
1
;
Keqi ZENG
1
;
Xin ZHOU
1
;
Yingfeng ZHANG
1
;
Cong NIE
1
Author Information
1. College of Chinese Materia Medica, Guangzhou University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
brain pharmacokinetics;
Buyang Huanwu Decoction;
cerebral ischemia;
LC-MS/MS;
microdialysis
- From:
Chinese Herbal Medicines
2022;14(4):630-637
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To conduct a comparative study on the brain pharmacokinetics of seven ingredients (i. e. senkyunolide A, ferulic acid, formononetin, calycosin, ononin, calycosin-O-β-D-glucopyranoside, and paeoniflorin), which were the compounds of Buyang Huanwu Decoction (BHD), in normal and cerebral ischemia rats administrated intragastrically with BHD. Methods: The samples of normal and permanent middle cerebral artery occlusion (pMCAO) rats were collected by using brain microdialysis technique. The concentrations of seven ingredients were determined by the HPLC-MS/MS method. After the BHD were administrated intragastrically to the rats for seven consecutive days, brain microdialysis probes were inserted into the hippocampus of rats, and then the brain microdialysates were collected at 20 min time intervals for 5 h. The separation of the seven ingredients and internal standard (IS) was carried out on an ACQUITY UPLC BEH C
