Establishment of large animal model of bicuspid aortic valve
- VernacularTitle:二叶式主动脉瓣畸形大动物模型的建立
- Author:
Quanlin YANG
1
;
Huan LIU
2
;
Yan SHAN
3
;
liming ZHU
2
;
Xiaoning SUN
2
;
Ye YANG
2
;
Wenshuo WANG
2
;
Lai WEI
2
;
Chunsheng WANG
2
Author Information
1. Department of Cardiac Surgery, Zhongshan-Xiamen Hospital, Fudan University, Xiamen, 361015, Fujian, P. R. China
2. Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China
3. Department of Medical Imaging, Zhongshan Hospital, Fudan University, Shanghai, 200032, P. R. China
- Publication Type:Journal Article
- Keywords:
Bicuspid aortic valve;
bicuspid aortopathy;
animal model;
wall shear stress
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2022;29(11):1485-1491
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the feasibility of establishment of animal model of "functional" bicuspid aortic valve with swine and observe its effect on the wall shear stress inside the aorta. Methods Four common Shanghai White Swine with body weight between 50 kg to 55 kg were selected. Under general anesthesia and cardiopulmonary bypass, the aortic transverse incision approach was used, continuous suture with 6-0 polypropylene to align the left and right coronary valve leaflets to create a bicuspid valve morphology. After the operation, echocardiography was used to observe the aortic valve morphology and the hemodynamic changes of the aortic valve orifice. The effect on the wall shear stress inside the aorta was studied with 4D-Flow magnetic resonance imaging (MRI). Results A total of 4 swine "functional" bicuspid aortic valve models were established, with a success rate of 100.0%. Echocardiography showed that the blood flow velocity of the aortic valve orifice was faster than that before the operation (0.96 m/s vs. 1.80 m/s). 4D-Flow MRI showed abnormally increased wall shear stress and blood flow velocity in the aorta of the animal models. After the surgery, in model animals, the maximal wall shear stress inside the ascending aorta was greater than 1.36 Pa, and the maximum blood flow velocity was greater than 1.4 m/s. Conclusion Establishment of the animal model of "functional" bicuspid aortic valve in swine is feasible, scientific and reliable. It can be used in researches on evaluating the pathophysiological changes.
