Effect of Adipose-derived Stem Cells Compound Chitosan Transplantation on Tumor Necrosis Factor-α, Interleukin-1β Content in Early Degenerate Intervertebral Disc of Rabbits
- VernacularTitle:脂肪干细胞复合壳聚糖支架移植对兔退变早期椎间盘内肿瘤坏死因子α、白介素1β的影响
- Author:
Jinzhen LI
;
Fang LI
;
Chaoqun YE
;
Dajiang REN
;
Zhongyuan WAN
;
Kun WU
- Publication Type:Journal Article
- Keywords:
intervertebral disc degeneration, tissue engineering, adipose-derived stem cells, chitosan, tumor necrosis factor-α, interleukin-1β
- From:
Chinese Journal of Rehabilitation Theory and Practice
2011;17(3):229-231
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of adipose-derived stem cells (ADSCs) compound injective thermo-sensitive chitosan scaffold transplantation on content of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) in early degenerate lumbar intervertebral disc of rabbits. Methods24 white New Zealand rabbits (no limit of male or female) were randomly and equally divided into 4 groups: A. Degeneration model group: nucleus aspiration. B. ADSCs compound chitosan transplantation group. C. Cell-free chitosan transplantation group. D. Blank control group: only explore the target intervertebral disc. When aspirate pulposus with 21G needle, inject ADSCs-scaffold complex and chitosan scaffold respectively. The samples of L2-3, L3-4, L4-5, L5-6 intervertebral disc were obtained from 2 rabit in each group 2, 4, 8 weeks after operation. The contents of TNF-α, IL-1β were measured with ELISA. ResultsAll animals survived after the operation. Compare with the blank control group, the contents of TNF-α, IL-1β in degeneration model group increased significantly (P＜0.05). It decreased significantly (P＜0.05) in ADSCs compound chitosan transplantation group and cell-free chitosan transplantation group compared to model group. IL-1β decreased significantly (P＜0.05) 8 week after operation in ADSCs compound chitosan transplantation group compared to cell-free chitosan transplantation group. ConclusionADSCs compound injective thermo-sensitive chitosan scaffold transplantation in early degenerate lumbar intervertebral disc could decrease the content of TNF-α, IL-1β, and may regulate the inflammatory response.
