Adherent Monoculture of Primary Neural Stem Cells Derived Fetal Rat Telencephalon
- VernacularTitle:胎鼠端脑神经干细胞原代单层化贴壁培养
- Author:
Jun LIANG
;
Xiaoyu SONG
;
Jianjiang DONG
;
Yuezhen LI
- Publication Type:Journal Article
- Keywords:
neural stem cells, cell culture, telencephalon, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2011;17(3):226-228
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo introduce a simple and quick method of adherent monoculture to primary neural stem cells depending on the mainly peptic characteristic of collagenase. MethodsThe fetus rat telencephalons of a pregnant 14-day-old SD rat were isolated, and tissues were treated with collagenase type Ⅰ contained EDTA following trituration. The cells were raised in polylysine culture plates with serum-free medium, and assessed with Nestin immunofluorescence. ResultsThe Nestin positve cells were obtained, and the purity was over 99%. ConclusionThis method may simply and quickly yields the primary neural stem cells that are monolayer and adherent.
