Effects of matrine combined with LY294002 on proliferation, apoptosis and cell cycle of human myeloid leukemia K562 cells.
10.12122/j.issn.1673-4254.2022.11.20
- Author:
Yan Mei HAO
1
;
Jun Li JI
1
;
Chun Yi LIU
1
;
Nan ZHANG
1
;
Ya Juan GONG
1
Author Information
1. Key Laboratory of Cancer Research and Clinical Laboratory Diagnosis, Bengbu Medical College, Bengbu 233030, China.
- Publication Type:Journal Article
- Keywords:
Akt;
LY294002;
acute myeloid leukemia;
apoptotic;
cell cycle;
matrine
- MeSH:
Humans;
K562 Cells;
Caspase 9;
Matrines;
Phosphatidylinositol 3-Kinases;
Cell Cycle;
Cell Division;
Leukemia, Myeloid;
Apoptosis;
Proto-Oncogene Proteins c-bcl-2
- From:
Journal of Southern Medical University
2022;42(11):1739-1746
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of matrine combined with LY294002 on proliferation, apoptosis and cell cycle of human myeloid leukemia K562 cells and explore the underlying mechanism.
METHODS:The effects of different concentrations of matrine alone and in combination with LY294002 on the proliferation of K562 cells were examined with CCK-8 assay. The changes in morphology of K562 cells were observed following treatment for 48 h with 0.4 g/L matrine and 10 μmol/L Y294002, either alone or in combination, and cell apoptosis was detected using flow cytometry with annexin V-FITC/PI double labeling; the changes in cell cycle was detected with PI labeling. Western blotting was performed to examine the effect of matrine combined with LY294002 on expressions of p-mTOR, p-PI3K, Akt, p-Akt, cyclinD1, Bcl-2 and caspase-9 in the cells.
RESULTS:Treatment with different concentrations of matrine, both alone and in combination with LY294002, inhibited the proliferation of K562 cells in a time- and concentration-dependent manner. Compared with matrine treatment alone, the combined treatment caused more obvious morphological changes of the cells, significantly increased cell apoptosis (P < 0.01), and induced cell cycle arrest in G0/G1 (P < 0.01). Western blotting showed that the protein expression levels of p-mTOR, cyclinD1, p-PI3K, p-Akt and Bcl-2 in K562 cells increased while the expression level of caspase-9 decreased significantly after the combined treatment (P < 0.01).
CONCLUSION:Matrine combined with LY294002 produces a synergistic inhibitory effect on K562 cells possibly by down-regulating the p-Akt expression in PI3K/Akt signaling pathway, reducing the expressions of p-mTOR, cyclinD1 and Bcl-2, and increasing the expression of caspase-9.
