Cuprous oxide nanoparticles-based photothermal and chemodynamic synergistic therapy inhibits proliferation and migration of gastric cancer cells in vitro.
10.12122/j.issn.1673-4254.2022.11.19
- Author:
Hai Fei WEN
1
;
Xian Ying HUANG
1
Author Information
1. Nanfang Hospital, First Clinical Medical College, Southern Medical University, Guangzhou 510515, China.
- Publication Type:Journal Article
- Keywords:
chemodynamic therapy;
cuprous oxide;
nanoparticles;
photothermal therapy
- MeSH:
Humans;
Stomach Neoplasms/therapy*;
Sincalide;
Nanoparticles;
Cell Proliferation
- From:
Journal of Southern Medical University
2022;42(11):1732-1738
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the physicochemical characterization of cuprous oxide (Cu2O) nanoparticles and assess its antitumor effect against gastric cancer cells in vitro.
METHODS:The morphology, particle size and Fenton-like properties of Cu2O nanoparticles were analyzed using transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta potential analysis and ultraviolet absorption spectroscopy. CCK-8 assay and Transwell experiments were used for evaluating the in vitro anti-tumor effect of the nanometers in gastric cancer cells.
RESULTS:The prepared Cu2O nanoparticles had a quasi-circular structure with a diameter of about 100 nm. The temperature of the nanoparticles increased from 25 to 50 ℃ after irradiation with near-infrared light (NIR, 0.5W/cm2) for 5 min. At a nearly neutral pH (pH=6.5), the nanoparticles catalyzed the generation of a large amount of reactive oxygen species (ROS). CCK-8 assay and Transwell experiment showed that Cu2O nanoparticles concentration-dependently inhibited the proliferation, invasion and migration of gastric cancer cells.
CONCLUSION:Cu2O nanoparticles have good photothermal and chemokinetic properties with a strong anti-tumor effect, and can potentially serve as a new therapeutic agent for gastric cancer treatment.
