MC1R is highly expressed in esophageal squamous cell carcinoma.
10.12122/j.issn.1673-4254.2022.10.16
- Author:
Xiao Shi ZHOU
1
;
Jiang CHANG
1
;
Li Xiong PENG
1
;
Xi Lin LIU
2
;
Fa Zheng YU
1
;
Jian Feng XU
3
;
Sha Sha ZHANG
1
;
Pan HU
1
;
Zeng Shan LIU
1
;
Guo Jun ZHANG
1
Author Information
1. State Key Laboratory for Zoonotic Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
2. China-Japan Union Hospital, Jilin University, Changchun 130033, China.
3. Panjin Center for Inspection and Testing, Panjin 124010, China.
- Publication Type:Journal Article
- Keywords:
MC1R;
clinicopathological parameters;
esophageal squamous cell carcinoma;
molecular biomarker
- MeSH:
Humans;
Esophageal Squamous Cell Carcinoma;
Esophageal Neoplasms/metabolism*;
Stomach Neoplasms;
Cell Line, Tumor;
Immunohistochemistry;
Cell Proliferation;
Gene Expression Regulation, Neoplastic
- From:
Journal of Southern Medical University
2022;42(10):1552-1559
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of MC1R in esophageal squamous cell carcinoma and its correlation with the clinicopathological parameters.
METHODS:We analyzed the expression of MC1R in esophageal cancer based on data from TCGA databse and examined its expression levels using RT-PCR and Western blotting in a human esophageal epithelial cell line BAr-T, human esophageal squamous cell carcinoma cell lines ECA109, KYSE30, KYSE150, KYSE510, TE-1, TE-13, and EC9706, a human gastric cancer cell line SGC7901 and 19 pairs of esophageal squamous cell carcinoma tissues and adjacent tissues.Immunohistochemistry was used to detect MC1R expression levels in 32 pairs of paraffin-embedded sections of esophageal squamous cell carcinoma and adjacent tissues, and the correlation of MC1R expression and the patients'clinicopathological characteristics was analyzed.
RESULTS:Bioinformatics analysis showed that MC1R was significantly overexpressed in esophageal cancer tissues (P < 0.05).MC1R expression was also increased in 5 esophageal squamous cell carcinoma cell lines ECA109, KYSE30, KYSE510, TE-13, EC9706 and the gastric cancer cell line SGC7901 as compared with that in esophageal epithelial cells (P < 0.05).Immunohistochemistry revealed significantly increased MC1R expression in esophageal squamous cell carcinoma tissue sections in comparison with the adjacent tissue sections (P < 0.05).In patients with esophageal squamous cell carcinoma, a high MC1R expression was detected mainly in those with an old age, positive for middle-thoracic involvement, and with moderately differentiated tumor cells, and showed a correlation with T stage of tumor (P < 0.05), but not with the other clinicopathological parameters such as gender, age, degree of cell differentiation, primary tumor site, or TNM stage (P>0.05).
CONCLUSION:MC1R is highly expressed in esophageal squamous cell carcinoma and may serve as a molecular biomarker to assist in the diagnosis of esophageal squamous cell carcinoma.
