microRNA let-7g-3p regulates proliferation, migration, invasion and apoptosis of bladder cancer cells by targeting HMGB2.
10.12122/j.issn.1673-4254.2022.09.09
- Author:
Zhen Hai ZOU
1
;
Qi CHENG
1
;
Zhong LI
1
;
Wu Yue GAO
1
;
Wei SUN
1
;
Bei Bei LIU
1
;
Yuan Yuan GUO
1
;
Jian Min LIU
1
Author Information
1. Department of Urology, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, China.
- Publication Type:Journal Article
- Keywords:
HMGB2;
bladder cancer;
let-7g-3p
- MeSH:
Apoptosis;
Cell Line, Tumor;
Cell Movement/physiology*;
Cell Proliferation;
Epithelial Cells/metabolism*;
Gene Expression Regulation, Neoplastic;
HMGB2 Protein/metabolism*;
Humans;
MicroRNAs/metabolism*;
Urinary Bladder;
Urinary Bladder Neoplasms/genetics*
- From:
Journal of Southern Medical University
2022;42(9):1335-1343
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the molecular mechanism by which microRNA let-7g-3p regulates biological behaviors of bladder cancer cells.
METHODS:The expression levels of let-7g-3p in bladder cancer and adjacent tissues, normal bladder epithelial cells (HUC cells) and bladder cancer cells (T24, 5637 and EJ cells) were detected using qRT- PCR. T24 cells were transfected with let-7g-3p mimic or inhibitor, and the changes in cell proliferation, migration, invasion, and apoptosis were examined. Transcriptome sequencing was carried out in cells overexpressing let-7g-3p, and the results of bioinformatics analysis, double luciferase reporter gene assay, qRT-PCR and Western blotting confirmed that HMGB2 gene was the target gene of let-7g-3p. The expression of HMGB2 was examined in HUC, T24, 5637 and EJ cells, and in cells with HMGB2 knockdown, the effect of let-7g-3p knockdown on the biological behaviors were observed.
RESULTS:qRT-qPCR confirmed that let-7g-3p expression was significantly lower in bladder cancer tissues and cells (P < 0.01). Overexpression of let-7g-3p inhibited cell proliferation, migration and invasion, and promoted cell apoptosis, while let-7g-3p knock-down produced the opposite effects. Bioinformatics and transcriptome sequencing results showed that HMGB2 was the key molecule that mediate the effect of let-7g-3p on bladder cancer cells. Luciferase reporter gene assay, qRT-PCR and Western blotting all confirmed that HMGB2 was negatively regulated by let-7g-3p (P < 0.01). Knocking down HMGB2 could partially reverse the effect of let-7g-3p knockdown on the biological behaviors of the bladder cancer cells.
CONCLUSION:The microRNA let-7g-3p can inhibit the biological behavior of bladder cancer cells by negatively regulating HMGB2 gene.
