Cold stress reduces lifespan and mobility of C. elegans by mediating lipid metabolism disorder and abnormal stress.
10.12122/j.issn.1673-4254.2022.08.07
- Author:
Hao SHI
1
;
Chao ZHANG
2
;
Jia Min ZHAO
1
;
Yi Wen LI
1
;
Yun Jia LI
1
;
Jun Jie LI
1
;
Zhi Yun ZENG
1
;
Lei GAO
1
Author Information
1. School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.
2. Department of Biochemistry, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China.
- Publication Type:Journal Article
- Keywords:
C. elegans;
cold stress;
lipid metabolism;
stress response
- MeSH:
Animals;
Antioxidants/metabolism*;
Caenorhabditis elegans;
Caenorhabditis elegans Proteins/genetics*;
Cold-Shock Response;
Lipid Metabolism;
Lipid Metabolism Disorders;
Longevity/genetics*
- From:
Journal of Southern Medical University
2022;42(8):1159-1165
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the changes of lipid metabolism and stress response of adult C.elegans exposed to non-freezing low temperature and explore the possible mechanism.
METHODS:The survival rate and activity of adult C.elegans cultured at 20℃ or 4℃ were observed.Lipid metabolism of the cultured adult C.elegans was evaluated using oil red O staining and by detecting the expressions of the genes related with lipid metabolism.The effects of low temperature exposure on stress level of adult C.elegans were evaluated using mitochondrial fluorescence staining and by detecting the expression levels of stress-related genes and antioxidant genes at both the mRNA and protein levels.
RESULTS:The lifespan and activity of adult C.elegans exposed to low temperature were significantly reduced with decreased lipid accumulation (P < 0.05) and decreased expressions of genes related with fatty acid synthesis and metabolism (fat-5, fat-6, fat-7, fasn-1, nhr-49, acs-2 and aco-1;P < 0.01).Cold stress significantly increased the expressions of heat shock proteins hsp-70 and hsp16.2(P < 0.05) but lowered the number of mitochondria (P < 0.0001) and the expressions of atfs-1, sod-2, sod-3 and gpx-1(P < 0.05).Knockout of fat-5, nhr-49 or both fat-5 and fat-6 obviously enhanced the sensitivity of C.elegans to cold stress as shown by further reduced activity (P < 0.05) and reduced survival rate at 24 h (P < 0.0001) under cold stress.
CONCLUSION:Exposure to a low temperature at 4℃ results in lowered lipid metabolism of adult C.elegans accompanied by a decreased mitochondrial number and quality control ability, which triggers high expressions of stress-related genes and causes reduction of antioxidant capacity, thus callsing lowered activity and reduced lifespan of C.elegans.
