Graphite furnace atomic absorption spectrometry for the determination of trace gallium in whole blood.
10.3760/cma.j.cn121094-20211018-00510
- VernacularTitle:全血中痕量镓测定的石墨炉原子吸收光谱法
- Author:
Le Zhou ZHOU
1
;
Lu YANG
1
Author Information
1. The Central Laboratory of Hunan Prevention and Treatment Institute for Occupational Disease, Changsha 410007, China.
- Publication Type:Journal Article
- Keywords:
Blood;
Gallium;
Graphite furnace atomic absorption;
Palladium chloride;
Spectrometry
- MeSH:
Spectrophotometry, Atomic/methods*;
Graphite/chemistry*;
Gallium;
Limit of Detection;
Temperature
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2022;40(10):776-778
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a graphite furnace atomic absorption spectrometry method for the determination of trace gallium in whole blood. Methods: From January to May 2021, the five factors of ashing temperature, ashing time, atomization temperature, atomization time and matrix modifier concentration in the determination of gallium in whole blood by graphite furnace atomic absorption spectrometry were optimized by using L(16) (4(5)) orthogonal test design. At the same time, within-run, between-run, spiking recovery test and other methodological indicators were tested. Results: Under the optimized detection conditions, the linear range of determination of gallium in whole blood by graphite furnace atomic absorption spectrometry was 0.29-100.00 μg/L (r=0.9991) . The within-run and between-run relative standard deviations (RSD) of repetitive measurement at 10.0, 50.0, 80.0 μg/L concentration levels were 2.3%-4.4% and 1.5%-3.6%, the recovery rate of spiking was 98.1%-103.8%, and the detection limit of the method was 0.13 μg/L. Conclusion: Graphite furnace atomic absorption spectrometry for the determination of trace gallium in whole blood is easy to operate, has a wide linear range, low detection limit, accurate and reliable results, which is suitable for occupational health examinations and the determination of acute gallium poisoning.
