Determination of chlorobenzene metabolite-p-chlorophenol in urine by solid phase extraction-gas chromatography.
10.3760/cma.j.cn121094-20210615-00287
- VernacularTitle:尿中氯苯代谢产物对氯苯酚测定的固相萃取-气相色谱法
- Author:
Peng WANG
1
;
Yi Yao CAO
1
;
Hong REN
1
;
Xiang Jing GAO
1
;
Qiu Liang XU
1
;
Zhen ZHOU
1
Author Information
1. Zhejiang Provincial Center for Disease Control and Prevention, Occupational Health and Radiation Protection Institute, Hangzhou 310051, China.
- Publication Type:Journal Article
- Keywords:
Chlorobenzene;
Chromatography, gas;
Metabolite;
Solid phase extraction;
Urine;
p-chlorophenol
- MeSH:
Acetonitriles;
Chlorophenols;
Chromatography, Gas;
Chromatography, High Pressure Liquid;
Hydrochloric Acid;
Solid Phase Extraction/methods*
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2022;40(9):703-706
- CountryChina
- Language:Chinese
-
Abstract:
Objective: A method to determine chlorobenzene metabolite-p-chlorophenol in urine by solid phase extraction-gas chromatography was established. Methods: In May 2021, the urine sample was hydrolyzed at 100 ℃ for 1.5 h with 2 ml concentrated hydrochloric acid. After cooling and filtering, the sample was enriched and purified by Oasis(®)MAX 6cc SPE column. Drip washing with 0.01 mol/L hydrochloric acid solution and elution with acetonitrile, the eluent was volumized to 5 ml with acetonitrile and determined by gas chromatography, and quantify by standard curve method. Results: Calibration curve of the method was linear within the range of 1.61-80.30 μg/ml and showed good linearity with r=0.9997, the regression equation was y=1.51602x-0.10234. The determination limit was 0.17 μg/ml, and the limit of quantitation was 0.55 μg/ml. Recovery rates were between 89.3%-104.4%, the relative standard deviation (RSD) of intra-day measurements ranged from 4.3% to 6.7%, and the RSD of inter-day measurements ranged from 4.5% to 6.7%. Conclusion: This method could optimize sample pretreatment, and eliminate the interference of impurities, which is sensitive, efficient and accurate for the determination of chlorobenzene metabolite-p-chlorophenol in urine.
