The effect of pregnant rats exposed to radio frequency electromagnetic field on the hippocampal morphology and nerve growth factor of offspring rats.
10.3760/cma.j.cn121094-20210607-00281
- Author:
Sheng Qing Yu ZHANG
1
;
Pan Pan DU
2
;
Xiao SHU
3
;
Hui Xin WU
3
;
Yun Zhen MU
3
;
Xi Nan WU
4
;
Yuan ZHANG
5
Author Information
1. School of Public Health, Kunming Medical University, Kunming 650500, China Kunming Panlong District People's Hospital (Medical Department) , Kunming 650500, China.
2. Community Health Service Center of Changle Square, Beilin District, Xi'an 710000.
3. School of Public Health, Kunming Medical University, Kunming 650500, China.
4. Graduate School, Kunming Medical University, Kunming 650500, China.
5. Biomedical Engineering Institute, Kunming Medical University, Kunming 650500, China.
- Publication Type:Journal Article
- Keywords:
Aberdeen;
Electromagnetic fields;
Fertile period;
Hippocampus;
Neurotrophin;
Radiofrequency;
Ultrastructure
- MeSH:
Animals;
Electromagnetic Fields;
Female;
Hippocampus;
Male;
Mice;
Nerve Growth Factor;
Pregnancy;
Rats;
Rats, Wistar;
Semen
- From:
Chinese Journal of Industrial Hygiene and Occupational Diseases
2022;40(9):656-660
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effects of exposure of pregnant rats to radio frequency electromagnetic field on the ultrastructure of hippocampus and the levels of obesity related protein (FTO) and nerve growth factor (NGF) in offspring rats. Methods: In September 2019, 36 healthy 7-week-old Wistar rats were selected, including 24 female rats (150-200 g) and 12 male rats (200-250 g) . The male and female mice were mated in the cage at 2: 1 ratio at 18: 00 every night. The smear results showed that the sperm was positive and the mating was successful. The day was regarded as the 0 day of pregnancy. Pregnant rats were randomly divided into 3 experimental groups and 3 control groups, with 4 rats in each group. The experimental group was exposed to 1 800 MHz, Wi-Fi and 1 800 MHz+Wi-Fi respectively, and the three control groups were exposed to virtual exposure. 12 hours a day for 21 days in three batches. After the end of exposure, the offspring of each group were raised for 7 weeks. The ultrastructural changes of the hippocampus were observed by transmission electron microscopy, the FTO level in the hippocampus was determined by Western blot, and the NGF level in the brain tissue was determined by ELISA. Results: Transmission electron microscopy showed that the nuclei of hippocampal tissue of female and male rats in the 1800 MHz group were slightly contracted, the cytoplasm was slightly edema, and the nuclei of male rats were obviously irregular. In the offspring of male and female rats in the Wi-Fi group, the nucleus of hippocampal tissue contracted seriously, the cell membrane was irregular, and the cytoplasm appeared obvious edema. In the 1800 MHz+Wi-Fi group, the nuclei of hippocampal tissue of both male and female offspring rats were severely contracted, the nuclear membrane was irregular, and the cytoplasm was severely edema. there was no significant difference in FTO level among the groups (P>0.05) . Compared with other groups, NGF content in hippocampus of offspring rats in the 1800 MHz+Wi-Fi group was significantly higher (P<0.05) . Conclusion: Exposure to radio frequency electromagnetic fields will damage the morphological structure of hippocampal tissue of offspring and stimulate the increase of NGF expression in the hippocampus.
