Standardization of clinical application of mass spectrometry method for measurement of vitamin D in capillary blood of children: a multicenter study.
10.3760/cma.j.cn112140-20220731-00689
- Author:
Luan Luan LI
1
;
Xiao Nan LI
2
;
Fei Yong JIA
3
;
Mei Zhu CHI
4
;
Zhi Hong WEN
5
;
Fan YANG
6
;
Yu Ning LI
7
;
Li Jun HA
8
;
Ying YANG
9
;
Xiao Ling LONG
10
;
Shuan Feng FANG
11
;
Lu XIE
12
;
Hui Feng ZHANG
13
;
Xiaodan YU
1
Author Information
1. Department of Developmental and Behavioral Pediatrics, Shanghai Children's Medical Center,Shanghai Jiao Tong University School of Medicine, Shanghai 200127,China.
2. Department of Child Health Care, Children's Hospital of Nanjing Medical University, Nanjing 210008, China.
3. Department of Developmental and Behavioral Pediatrics, the First Hospital of Jilin University, Changchun 130021, China.
4. Department of Child Health Care, the First Hospital of Wenzhou Medical University, Wenzhou 325000, China.
5. Department of Pediatrics, the People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China.
6. Department of Child Health Care, West China Second University Hospital, Sichuan University,Chengdu 610041, China.
7. Department of Pediatrics, the First Hospital of Lanzhou University, Lanzhou 730013, China.
8. Department of Child Health Care, Yinchuan Maternity and Child Health Care Hospital, Yinchuan 750000, China.
9. Department of Child Health Care and Neurological Rehabilitation, Sports Center Branch of Dalian Municipal Women and Children's Medical Center (Group), Dalian 116033, China.
10. Department of Pediatrics, Boai Hospital of Zhongshan, Zhongshan 528405, China.
11. Department of Child Health Care, Children's Hospital of Zhengzhou University, Zhengzhou 450007, China.
12. Department of Pediatrics, Kunming Maternity and Child Health Care Hospital, Kunming 650031, China.
13. Department of Pediatrics, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China.
- Publication Type:Journal Article
- MeSH:
Child;
Humans;
Vitamin D;
Cross-Sectional Studies;
Tandem Mass Spectrometry;
Vitamins;
Reference Standards
- From:
Chinese Journal of Pediatrics
2022;60(12):1282-1287
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the norms and clinical application standards of mass spectrometry method to measure vitamin D in capillary blood. Methods: Following the "Province-City-Hospital" sampling procedure, a cross-sectional sample of 1 655 healthy children under 7 years of age were recruited from 12 provinces, autonomous regions, or municipalities in China from November 2020 to December 2021. Both venous and capillary blood samples from the same individual were collected, for which serum 25(OH)D levels were measured by high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) method. Pearson correlation analysis and linear regression analysis were used to detect the correlation and determine a correction algorithm. The agreement was analyzed using Bland-Altman plot and Kappa statistic. The sensitivity and specificity were evaluated using receiver operating characteristic (ROC) curve method. Results: Venous and capillary 25(OH)D levels of 1 655 healthy children under 7 years of age were 74.25 (59.50, 92.00) and 68.75 (54.44, 86.25) nmol/L, respectively, showed a significant difference(Z=22.14, P<0.001) as well as a highly significant correlation between venous and capillary 25(OH)D levels(r=0.95, P<0.001). Linear regression analysis was then performed to determine the correction algorithm: lg(corrected capillary 25(OH)D)=0.13+0.95×lg(capillary 25(OH)D)(R2=0.90,P<0.001). The deviation between venous and corrected capillary 25(OH)D levels was (0.50±17.50) nmol/L, a difference value that did not reach statistical significance (P>0.05). The cut-off values of capillary blood 25(OH)D values 30.00, 50.00, 75.00 nmol/L corresponding to venous blood 25(OH)D values were 26.59, 45.56, and 69.84 nmol/L, respectively. Good consistency was observed between venous and corrected capillary 25(OH)D levels in clinical diagnosis (Kappa value 0.68-0.81). Corrected capillary 25(OH)D showed a high clinically predictive value (area under curve 0.97-0.99,sensitivity 0.72-0.92,specificity 0.89-0.99). Conclusion: The standardized capillary HPLC-MS/MS method can be used to detect 25(OH)D levels in children clinically.
